CAJ | 학술논문

背景:随着年龄的增加,脑内超氧化物歧化酶活性降低、丙二醛含量增加而造成的损伤作用越来越受到重视;猪脑肽溶液能否通过血脑屏障进入脑组织对脑神经起保护作用,目前只是临床病例报道,对其实验研究国、内外报道较少.目的:探讨猪脑肽溶液对D-半乳糖拟衰老小鼠脑以及肝脏组织中丙二醛活性和超氧化物歧化酶含量的影响.设计:以实验动物为研究对象的随机对照实验观察.单位:解放军总医院老年医学研究所.材料:实验于2001-02/2001-04在解放军总医院老年医学研究所完成(军队重点实验室).选择NH小鼠82只,雌雄各半,解放军总医院医学实验动物中心(清洁级)提供,体质量18～22 g,随机分为对照组、猪脑肽溶液(施普善)大、中、小剂量组、阿尼西坦组(阳性对照组)和D-半乳糖组.方法:各组分别给药,连续给6周,采用D-半乳糖诱导的脑老化小鼠动物模型.各组分别用化学发光法测定超氧化物歧化酶活性,用硫代巴比妥酸比色法测定MDA含量.主要观察指标:各组小鼠的SOD活性和丙二醛含量.结果:猪脑肽溶液大、中剂量均能显著提高脑以及肝脏组织中超氧化物歧化酶活性[分别为(30.23±5.23),(29.65±4.88);(19.84±5.79),(16.75±5.32)μkat/g],降低丙二醛含量[分别为(630.00±59.40),(626.74±49.68);(934.90±207.50),(916.06±235 31)nmol/g],与D-半乳糖组比较[分别为(22.89±4.54),(12.26±6.31)μkat/g;(749.69±92.80),(1 212.89±142.54)nmol/g],差异均有显著性意义(P＜0.05或P＜0.01).结论:猪脑肽溶液具有明显的抗氧化能力,对衰老小鼠脑神经细胞及肝细胞有一定的保护作用,为评估其对老年机体脑及肝细胞的抗自由基氧化损伤作用提供理论支持.
배경:수착년령적증가,뇌내초양화물기화매활성강저、병이철함량증가이조성적손상작용월래월수도중시;저뇌태용액능부통과혈뇌병장진입뇌조직대뇌신경기보호작용,목전지시림상병례보도,대기실험연구국、내외보도교소.목적:탐토저뇌태용액대D-반유당의쇠로소서뇌이급간장조직중병이철활성화초양화물기화매함량적영향.설계:이실험동물위연구대상적수궤대조실험관찰.단위:해방군총의원노년의학연구소.재료:실험우2001-02/2001-04재해방군총의원노년의학연구소완성(군대중점실험실).선택NH소서82지,자웅각반,해방군총의원의학실험동물중심(청길급)제공,체질량18～22 g,수궤분위대조조、저뇌태용액(시보선)대、중、소제량조、아니서탄조(양성대조조)화D-반유당조.방법:각조분별급약,련속급6주,채용D-반유당유도적뇌노화소서동물모형.각조분별용화학발광법측정초양화물기화매활성,용류대파비타산비색법측정MDA함량.주요관찰지표:각조소서적SOD활성화병이철함량.결과:저뇌태용액대、중제량균능현저제고뇌이급간장조직중초양화물기화매활성[분별위(30.23±5.23),(29.65±4.88);(19.84±5.79),(16.75±5.32)μkat/g],강저병이철함량[분별위(630.00±59.40),(626.74±49.68);(934.90±207.50),(916.06±235 31)nmol/g],여D-반유당조비교[분별위(22.89±4.54),(12.26±6.31)μkat/g;(749.69±92.80),(1 212.89±142.54)nmol/g],차이균유현저성의의(P＜0.05혹P＜0.01).결론:저뇌태용액구유명현적항양화능력,대쇠로소서뇌신경세포급간세포유일정적보호작용,위평고기대노년궤체뇌급간세포적항자유기양화손상작용제공이론지지.
BACKGROUND: More attention has been paid upon the injury effect resulted from the decrease of superoxide dismutase(SOD) activity and increase of malonaldehyde (MDA) content in brain. Whether pig brain peptide solution can pass blood-brain barrier and penetrate into brain tissue and protect brain nerve is still found in limited cases reports. Relevant reports are still rare both in China and abroad.OBJECTIVE: To explore the effect of pig brain peptide solution on SOD activity and MDA content in brain and liver of senile model mice made by D-galactose.DESIGN: Experimental animals-based randomized and controlled experimental observation.SETTING: Institute of Senile Medical of General Hospital of Chinese PLA.MATERIALS: The experimant was made in the Institute of Senile Medical (Chinese PLA key laboratory) of General HosPital of Chinese PLA from February to April 2001. Totally 82 NH mice, weighted from 18 to 22 g, half male and half female, were provided by the Medical Experimental Animal Center of General Hospital of Chinese PLA. These mice were randomly divided into the control group, hige dose group, middle dose group, low dose group, aniracetam group (positive control group), and D-galactose group.METHODS: Each group was given drugs seperately for 6 weeks. The senile mice model was induced by D-galactose. SOD activity of each group was checked with chemiluminescence method and MDA content examined with thiobarbituric acid colourimetry method.MAIN OUTCOME MEASURES: SOD activity and MDA content of mice in each group.RESULTS: Pig brain peptide solution with high and middle doses significantly increased SOD activity[ (30.23 ± 5.23), (29.65 ± 4. 88 );(19. 84 ± 5.79), (16.75 ± 5.32) μkat/g respectively], and decreased MDA content[ (630.00 04; 8(34): 7748-50CONCLUSION: Pig brain peptide solution has obvious antioxidative effect, and has certain protective effects on brain nerve cells and liver cells of senile mice.