CAJ | 학술논문

报道了水解酶催化二乙基锌与芳香醛的加成反应,对反应条件进行了优化.在最适条件(嗜热酯酶APE1547为酶源,反应温度40℃,氯仿为溶剂,4-Cl-苯甲醛为底物)下,加成反应生成的光学活性醇产率最高达78%,ee值最高可达56%.通过实验结果和分子动力学分析对可能的反应机理进行了推测.本研究进一步拓展了酶的非专一性.
보도료수해매최화이을기자여방향철적가성반응,대반응조건진행료우화.재최괄조건(기열지매APE1547위매원,반응온도40℃,록방위용제,4-Cl-분갑철위저물)하,가성반응생성적광학활성순산솔최고체78%,ee치최고가체56%.통과실험결과화분자동역학분석대가능적반응궤리진행료추측.본연구진일보탁전료매적비전일성.
A novel application of hydrolase as catalyst for the addition of diethylzinc to aromatic aldehydes iS reported.Optimum conditions were screened out.Under the optimum conditions(i.e.,reaction at 40 ℃ using hyperthermophilice esterase APE1547,4-Cl-benzaldehyde substrate,and trichloromethane solvent),this reaction provided optically active secondary alcohol with an enantiomeric excess up to 56% and a yield up to 78%.The possible mechanism was proposed based on experimental observations and molecular dynamics simulation.These observations extend the phenomenon of enzyme promiscuity.