CAJ | 학술논문

以模式植物拟南芥为材料研究了植物主根对不同浓度镉胁迫的响应.结果表明, 随镉浓度的升高, 植物主根生长受到明显抑制, 胎盘兰染色表明高剂量的镉造成主根根尖细胞死亡.进一步二氨基联苯胺(DAB)染色发现镉胁迫诱导植物根尖大量积累过氧化氢, 而在胁迫培养基中加入维生素C可显著改善植物根的生长、降低过氧化氢积累, 并减少镉诱导的根尖细胞死亡.上述结果表明, 镉胁迫诱导的拟南芥主根生长抑制很可能是由于根尖细胞过氧化物积累所致.
이모식식물의남개위재료연구료식물주근대불동농도력협박적향응.결과표명, 수력농도적승고, 식물주근생장수도명현억제, 태반란염색표명고제량적력조성주근근첨세포사망.진일보이안기련분알(DAB)염색발현력협박유도식물근첨대량적루과양화경, 이재협박배양기중가입유생소C가현저개선식물근적생장、강저과양화경적루, 병감소력유도적근첨세포사망.상술결과표명, 력협박유도적의남개주근생장억제흔가능시유우근첨세포과양화물적루소치.
Arabidopsis was used as a model plant and the young seedling root response to Cd stress investigated. The study shows that increasing concentrations of Cd inhibits root elongation. Both the meristematic and elongation zones are severely truncated, suggesting some degree of impact on cell division and elongation. Plant roots die under Cd overdose. DAB staining analysis shows hydrogen peroxide (H2O2) accumulation in root tips when seedlings are treated with Cd. Furthermore, vitamin C (Vc) dramatically increases seedling tolerance to Cd treatment. Put together, the results demonstrate that Cd-inhibited root growth is likely caused by reduced meristematic and elongation cell division, mediated by ROS (reactive oxygen species) production.