CAJ | 학술논문

目的探讨温肺化饮方治疗慢性阻塞性肺疾病(COPD)的机制.方法采用改良烟熏加气管滴加脂多糖方法复制COPD模型,二次造模复制成寒痰蕴肺型COPD.然后随机分为治疗组、模型组,治疗组灌服温肺化饮方,连续14天;模型组大鼠每天予2 mL生理盐水灌胃;另设空白对照组不做任何干预.观察大鼠一般情况,体重增长指数,光学显微镜下观察肺组织形态学改变,电子显微镜观察超微结构,以及大鼠动脉血气分析,放射免疫法检测肺组织匀浆白细胞介素(IL)4、IL-8、肿瘤坏死因子-α(TNF-α)、γ干扰素(IFN-γ)含量.结果治疗组大鼠扎堆恶寒现象、哮鸣音等症状及各级气道上皮细胞变性坏死、慢性炎性细胞浸润程度较模型组明显减轻;纤毛倒伏、脱落及肺大泡数量较模型组显著减少;治疗组大鼠体重增长指数较模型组显著上升(P<0.01);治疗组降低动脉血二氧化碳方压(PaCO_2)、IL-4、IL-8、TNF-α水平,升高动脉血氧分压(PaO_2)、IFN-γ水平及IFN-γ//IL-4比值明显优于模型组(P<0.01,P<0.05).结论温肺化饮方能纠正Th_1/Th_2比值的失衡,抑制炎性反应,对于COPD大鼠气道功能、结构改善起着非常重要的作用.
목적 탐토온폐화음방치료만성조새성폐질병(COPD)적궤제.방법 채용개량연훈가기관적가지다당방법복제COPD모형,이차조모복제성한담온폐형COPD.연후수궤분위치료조、모형조,치료조관복온폐화음방,련속14천;모형조대서매천여2 mL생리염수관위;령설공백대조조불주임하간예.관찰대서일반정황,체중증장지수,광학현미경하관찰폐조직형태학개변,전자현미경관찰초미결구,이급대서동맥혈기분석,방사면역법검측폐조직균장백세포개소(IL)4、IL-8、종류배사인자-α(TNF-α)、γ간우소(IFN-γ)함량.결과 치료조대서찰퇴악한현상、효명음등증상급각급기도상피세포변성배사、만성염성세포침윤정도교모형조명현감경;섬모도복、탈락급폐대포수량교모형조현저감소;치료조대서체중증장지수교모형조현저상승(P<0.01);치료조강저동맥혈이양화탄방압(PaCO_2)、IL-4、IL-8、TNF-α수평,승고동맥혈양분압(PaO_2)、IFN-γ수평급IFN-γ//IL-4비치명현우우모형조(P<0.01,P<0.05).결론 온폐화음방능규정Th_1/Th_2비치적실형,억제염성반응,대우COPD대서기도공능、결구개선기착비상중요적작용.
Objective To explore the mechanism of Wenfei Huayin Recipe (WHR) in treating chronic obstructive pulmonary diseases (COPD). Methods The COPD model was induced by modified fumigating method and intra-tracheal instillation of lipopolysaccharide. Then reformed COPD model of cold-phlegm retention in Fei syndrome type. All the model rats were randomly divided into two groups, the model group and the treated group, treated respectively with WHR and saline for 14 successive days. Besides, a blank group without any intervention was set up for control. The general condition, weight growth rate, pathological changes of lung tissue under light microscope, ultrastructure under electron microscope, arterial blood gas analysis and levels of interleukin 4 (IL-4), interleukin 8 (IL-8), tumor necrosis factor α (TNF-α) and interferon γ (IFN-γ) in lung homogenate by radio-immunity assay were observed. Results In the treated group, as compared with the control group, the symptoms of aversion to cold, swarming, wheezing, the degree of epithelial cell degeneration and necrosis, the inflammatory cell infiltration and the volume of cilia lodging, sloughing, and bullae of lung were lessened and weight growth rate was higher (P<0.01). Moreover, the treated group was superior to the control group in decreasing levels of PaCO_2, IL-4, IL-8, TNF-α and increasing PaO_2, IFN-γ and IFN-γ/IL-4 ratio (P<0.01 or P<0.05). Conclusion WHR can correct the Th_1/Th_2 imbalance and inhibit the inflammatory reaction, displaying an important role in improving the airway function and structure in COPD rats.