食品科学
食品科學
식품과학
FOOD SCIENCE
2010年
3期
198-202
,共5页
马克斯克鲁维酵母%菊粉酶%纯化%酶学性质
馬剋斯剋魯維酵母%菊粉酶%純化%酶學性質
마극사극로유효모%국분매%순화%매학성질
Kluyveromyces marxianus%inulinase%purification%enzymatic property
马克斯克鲁维酵母产生的孢外菊粉酶经超滤浓缩、DEAE-Cellulose阴离子交换色谱、SephadexG-100凝胶色谱分离纯化,得到两个菊粉酶组分Exo Ⅰ和Exo Ⅱ,I/S值分别为0.0249和0.0253,均属外切菊粉酶.Exo Ⅰ经聚丙烯酰胺凝胶鉴定为均一组分,分子量为85kD.Exo Ⅰ最适pH值为4.0,最适温度为60℃,Mn~(2+)和Mg~(2+)对酶活力有促进作用,而Cu~(2+)、Fe~(2+)对酶活力有抑制作用,Exo Ⅰ水解菊粉溶液的产物为果糖和少量葡萄糖.
馬剋斯剋魯維酵母產生的孢外菊粉酶經超濾濃縮、DEAE-Cellulose陰離子交換色譜、SephadexG-100凝膠色譜分離純化,得到兩箇菊粉酶組分Exo Ⅰ和Exo Ⅱ,I/S值分彆為0.0249和0.0253,均屬外切菊粉酶.Exo Ⅰ經聚丙烯酰胺凝膠鑒定為均一組分,分子量為85kD.Exo Ⅰ最適pH值為4.0,最適溫度為60℃,Mn~(2+)和Mg~(2+)對酶活力有促進作用,而Cu~(2+)、Fe~(2+)對酶活力有抑製作用,Exo Ⅰ水解菊粉溶液的產物為果糖和少量葡萄糖.
마극사극로유효모산생적포외국분매경초려농축、DEAE-Cellulose음리자교환색보、SephadexG-100응효색보분리순화,득도량개국분매조분Exo Ⅰ화Exo Ⅱ,I/S치분별위0.0249화0.0253,균속외절국분매.Exo Ⅰ경취병희선알응효감정위균일조분,분자량위85kD.Exo Ⅰ최괄pH치위4.0,최괄온도위60℃,Mn~(2+)화Mg~(2+)대매활력유촉진작용,이Cu~(2+)、Fe~(2+)대매활력유억제작용,Exo Ⅰ수해국분용액적산물위과당화소량포도당.
Crude extracellular inulinase from Kluyveromyces marxianus was purified through sequential steps of ultrafiltration,DEAE-cellulose anion exchange chromatography and SephadexG-100 gel filtration to obtain two components,designated as Exo Ⅰand ExoⅡ inulinase.The molecular weight of Exo Ⅰ inulinase was 85kD.The optimal reaction pH and temperature of Exo Ⅰwere 4.0 and 60℃,respectively.In addition,Mn~(2+) and Mg~(2+) could improve the activity of Exo Ⅰinulinase;in contrast,Cu~(2+) and Fe~(2+) exhibited a strong inhibition effect on Exo Ⅰinulinase activity.Enzymatic hydrolysates of inulin by Exo Ⅰ inulinase were fructose and a small amount of glucose.
