植物生态学报
植物生態學報
식물생태학보
ACTA PHYTOECOLOGICA SINICA
2010年
4期
438-443
,共6页
非共生萌发%沼兰%正交设计%冗余分析%种子形态
非共生萌髮%沼蘭%正交設計%冗餘分析%種子形態
비공생맹발%소란%정교설계%용여분석%충자형태
asymbiotic germination%Malaxis monophyllos%orthogonal test%redundancy analysis%seed morphology
近年来由于灭绝性采挖和生态环境的恶化,沼兰(Malaxis monophyllos)的自然资源遭到严重破坏,加上沼兰种子具有萌发缓慢和萌发率低的特性,已处于近危乃至易危的状态.为了繁殖和保护沼兰种质资源,利用添加2%蔗糖、3%活性炭和0.8%琼脂的Knudson C(KC)培养基,研究了萘乙酸(NAA)、6-苄基腺嘌呤(6-BA)、椰乳、香蕉泥、土豆泥和光照条件6种因素对沼兰种子非共生萌发的影响;同时通过光学显微镜和扫描电镜观察了种子的外部形态.结果显示,沼兰种皮细胞的平周壁方向结构平坦、没有纹络,但是沿着细胞垂周壁方向细胞壁较厚.通过正交试验设计和冗余分析,建立了沼兰种子非共生萌发的最佳方案,即在含有4.5 mg·L~(-1)NAA、10 mg·L~(-1)6-BA、8%香蕉泥和3%土豆泥的KC培养基上光照条件(光照强度为20μmol·m~(-2)·s~(-1),光周期为12 h/12 h)下萌发.该方案的种子萌发率在90%以上.
近年來由于滅絕性採挖和生態環境的噁化,沼蘭(Malaxis monophyllos)的自然資源遭到嚴重破壞,加上沼蘭種子具有萌髮緩慢和萌髮率低的特性,已處于近危迺至易危的狀態.為瞭繁殖和保護沼蘭種質資源,利用添加2%蔗糖、3%活性炭和0.8%瓊脂的Knudson C(KC)培養基,研究瞭萘乙痠(NAA)、6-芐基腺嘌呤(6-BA)、椰乳、香蕉泥、土豆泥和光照條件6種因素對沼蘭種子非共生萌髮的影響;同時通過光學顯微鏡和掃描電鏡觀察瞭種子的外部形態.結果顯示,沼蘭種皮細胞的平週壁方嚮結構平坦、沒有紋絡,但是沿著細胞垂週壁方嚮細胞壁較厚.通過正交試驗設計和冗餘分析,建立瞭沼蘭種子非共生萌髮的最佳方案,即在含有4.5 mg·L~(-1)NAA、10 mg·L~(-1)6-BA、8%香蕉泥和3%土豆泥的KC培養基上光照條件(光照彊度為20μmol·m~(-2)·s~(-1),光週期為12 h/12 h)下萌髮.該方案的種子萌髮率在90%以上.
근년래유우멸절성채알화생태배경적악화,소란(Malaxis monophyllos)적자연자원조도엄중파배,가상소란충자구유맹발완만화맹발솔저적특성,이처우근위내지역위적상태.위료번식화보호소란충질자원,이용첨가2%자당、3%활성탄화0.8%경지적Knudson C(KC)배양기,연구료내을산(NAA)、6-변기선표령(6-BA)、야유、향초니、토두니화광조조건6충인소대소란충자비공생맹발적영향;동시통과광학현미경화소묘전경관찰료충자적외부형태.결과현시,소란충피세포적평주벽방향결구평탄、몰유문락,단시연착세포수주벽방향세포벽교후.통과정교시험설계화용여분석,건립료소란충자비공생맹발적최가방안,즉재함유4.5 mg·L~(-1)NAA、10 mg·L~(-1)6-BA、8%향초니화3%토두니적KC배양기상광조조건(광조강도위20μmol·m~(-2)·s~(-1),광주기위12 h/12 h)하맹발.해방안적충자맹발솔재90%이상.
Aims Extensive collection, environmental deterioration, slow seed germination and low germination percentage have seriously degraded the natural resources of Malaxis monophyllos, resulting in a vulnerable situation for the species. Our objective is to establish an asymbiotic germination system of M. monophyllos seeds to protect and propagate the species.Methods Seed morphology was investigated by optical microscope and scanning electron microscope. We used Knudson C medium supplemented with 2% sucrose, 3% activated charcoal and 0.8% agar as the basic medium to investigate the effects of a-naphthalene acetic acid (NAA), 6-benzylaminopurine (6-BA), coconut milk, meshed banana, meshed potato and light on seed germination via the orthogonal test. Important findings Periclinal walls of testa cells were smooth and had no veins, but anticlinal walls were thick and had clear surface patterns. Through the orthogonal test and redundancy analysis, an optimal formula for asymbiotic seed germination was determined: the medium supplemented with 4.5 mg·L~(-1) NAA, 10 mg·L~(-1) 6-BA,8% meshed banana and 3% meshed potato in the light (12 h light/12 h dark at 20 μmol·m~(-2)·s~(-1) of light intensity).Seed germination was > 90% with this formula.
