CAJ | 학술논문

本文采用MTT法,观察不同浓度辛伐他汀(0,1,3,10,30 and 100 μmol/L)对C2C12细胞活力的影响,并通过定量RT-PCR方法对utrophin和TNF-α基因的mRNA的表达进行了研究.随着辛伐他汀浓度和作用时间的增加,C2C12细胞的活力在降低；不同浓度辛伐他汀对utrophin和TNF-α的mRNA表达具有影响作用,为阐明辛伐他汀的肌毒性作用机理提供了新的思路.
본문채용MTT법,관찰불동농도신벌타정(0,1,3,10,30 and 100 μmol/L)대C2C12세포활력적영향,병통과정량RT-PCR방법대utrophin화TNF-α기인적mRNA적표체진행료연구.수착신벌타정농도화작용시간적증가,C2C12세포적활력재강저；불동농도신벌타정대utrophin화TNF-α적mRNA표체구유영향작용,위천명신벌타정적기독성작용궤리제공료신적사로.
The effects of simvastatin on the cell viability and the mRNA expressed state of utrophin gene in C2C12 cells were measured.Simultaneously,the mRNA of tumor necrosis factor α (TNF-α) was examined to get the information of inflammatory cytokines after simvastatin treated C2C12 cells.C2C12 cells were treated by 0,1,3,10,30 and 100 μmol/L simvastatin for 24 h and 48 h.MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay was used to test cell viability.The real-time quantitative RT-PCR was used to test mRNA expression.Simvastatin inhibits the cell growth with the concentration increase and effect mRNA levels of utrophin and TNF-α in C2C12 cell line.These results might be helpful to elucidate the mechanism of side effects occur in skeletal muscle induced by simvastatin.