CAJ | 학술논문

目的探讨免疫抑制剂对小鼠肺泡巨噬细胞抗曲霉感染相关受体mRNA表达的影响.方法 30只昆明小鼠随机分为2组:正常对照组(6只)和免疫抑制组(24只,环磷酰胺150 mg/kg腹腔注射).免疫抑制组小鼠注射环磷酰胺后4 h、8 h、16 h及24 h,分别随机取6只进行支气管肺泡灌洗,收集肺泡巨噬细胞.正常对照组小鼠注射生理盐水24 h后处死,收集肺泡巨噬细胞.使用逆转录-聚合酶链反应检测小鼠肺泡巨噬细胞Toll样受体2(TLR2)、TLR4、树突状细胞相关C型凝集素1(Dectine-1)mRNA表达变化.结果与正常对照组比较,腹腔注射环磷酰胺4 h后,TLR2 mRNA表达即出现显著下降(P＜0.01);在腹腔注射环磷酰胺8 h后TLR4 mRNA表达出现显著下降(P＜0.01);Dectine-1 mRNA在腹腔注射环磷酰胺后无明显变化.结论免疫抑制剂环磷酰胺能够下调肺泡巨噬细胞抗曲霉感染相关受体TRL2和TLR4 mRNA的表达,对Dectine-1 mRNA的表达未见明显影响.
목적 탐토면역억제제대소서폐포거서세포항곡매감염상관수체mRNA표체적영향.방법 30지곤명소서수궤분위2조:정상대조조(6지)화면역억제조(24지,배린선알150 mg/kg복강주사).면역억제조소서주사배린선알후4 h、8 h、16 h급24 h,분별수궤취6지진행지기관폐포관세,수집폐포거서세포.정상대조조소서주사생리염수24 h후처사,수집폐포거서세포.사용역전록-취합매련반응검측소서폐포거서세포Toll양수체2(TLR2)、TLR4、수돌상세포상관C형응집소1(Dectine-1)mRNA표체변화.결과 여정상대조조비교,복강주사배린선알4 h후,TLR2 mRNA표체즉출현현저하강(P＜0.01);재복강주사배린선알8 h후TLR4 mRNA표체출현현저하강(P＜0.01);Dectine-1 mRNA재복강주사배린선알후무명현변화.결론 면역억제제배린선알능구하조폐포거서세포항곡매감염상관수체TRL2화TLR4 mRNA적표체,대Dectine-1 mRNA적표체미견명현영향.
Objective To investigate the effects of immunosuppressive agent on mRNA expression of anti-aspergillus infection-associated receptors on alveolar macrophage.Methods Thirty Kunming mice were randomly divided into two groups:normal control group(n=6)and immunocompromise group(150 mg/kg cyclophosphamide,intraperitoneal injection,n=24).Six mice were randomly taken to collect alveolar macrophage by bronchoalveolar lavage from immunocompromise group at time of 4,8,12 and 24 hours after immunosuppression.Mice in normal control group were sacrificed to collect alveolar macrophage after receiving normal saline intraperitoneal injection.Reverse transcription-polymerase chain reaction was used for determining the mRNA expression of Toll-like receptor 2(TLR2),TLR4 and dendritic cell-associated C-type lectin 1(Dectine-1).Results TLR2 mRNA expression decreased signiffieantly four hours after cyclophosphamide intraperitoneal injection,and decreased continually to 24 hours(all P＜0.01).The decrease of TLR4 mRNA expression appeared eight hours after immunosuppression,and decreased continually to 24 hours(all P＜0.0 1).There was not significant difference on Dectine-1 mRNA expression between normal control group and immunocompromise group.Conclusions The results suggest that cyclophosphamide could down-regulate the mRNA expression of TLR2 and TLR4 on alveolar maerophage.