CAJ | 학술논문

目的探索应用骺板组织工程治疗骺板损伤的新方法.方法将骺板软骨细胞与可降解多孔丝素材料复合,植入骺板损伤的动物模型中,同时设单纯植入可降解多孔丝素材料组和空白对照组.应用组织学Mason三色染色、细胞学BrdU细胞示踪技术、影像学X线检测等方法,观察骺板修复、短缩畸形和成角畸形情况.结果通过Masson三色染色我们发现:骺板软骨细胞复合可降解多孔丝素材料组(SF+ cell)存在一定的短缩畸形和成角畸形,骺板损伤处发现类似正常的柱状排列细胞；单纯植入可降解多孔丝素材料组(SF)存在明显的短缩畸形和成角畸形,骺板损伤处发现松散的柱状排列细胞；空白对照组存在显著的短缩畸形和成角畸形,骺板损伤处未见柱状排列细胞.通过X线检测我们发现:三组短缩畸形分别为(3.24±0.58)％、(6.32±0.37)％和(8.47±0.51)％.三组成角畸形分别为(4.89±1.27)°、(10.67±1.58)°和(18.44±2.65)°.其短缩畸形和成角畸形的程度均存在统计学意义(均P=0.0001,P＜0.01).结论骺板组织工程方法治疗骺板损伤是一种可行的方法,骺板软骨细胞复合可降解多孔丝素材料组的疗效优于单纯植入可降解多孔丝素材料组.
목적 탐색응용후판조직공정치료후판손상적신방법.방법 장후판연골세포여가강해다공사소재료복합,식입후판손상적동물모형중,동시설단순식입가강해다공사소재료조화공백대조조.응용조직학Mason삼색염색、세포학BrdU세포시종기술、영상학X선검측등방법,관찰후판수복、단축기형화성각기형정황.결과 통과Masson삼색염색아문발현:후판연골세포복합가강해다공사소재료조(SF+ cell)존재일정적단축기형화성각기형,후판손상처발현유사정상적주상배렬세포；단순식입가강해다공사소재료조(SF)존재명현적단축기형화성각기형,후판손상처발현송산적주상배렬세포；공백대조조존재현저적단축기형화성각기형,후판손상처미견주상배렬세포.통과X선검측아문발현:삼조단축기형분별위(3.24±0.58)％、(6.32±0.37)％화(8.47±0.51)％.삼조성각기형분별위(4.89±1.27)°、(10.67±1.58)°화(18.44±2.65)°.기단축기형화성각기형적정도균존재통계학의의(균P=0.0001,P＜0.01).결론 후판조직공정방법치료후판손상시일충가행적방법,후판연골세포복합가강해다공사소재료조적료효우우단순식입가강해다공사소재료조.
Objective To explore the effect of tissue engineered epiphyseal plate on epiphyseal plate injury.Methods The engineered epiphyseal plate and scaffold were respectively implanted to the animal model of epiphyseal plate injury,Masson staininng and BrdU tracing assay,as well X-ray were utilized for evaluation of efficacy.Results The Mason staining indicated reduced deformity degree in engineered epiphyseal plate group compared with scaffold group,whereas the deformity degree in scaffold group was less in contrast to the control group.The X-ray results showed that in engineered epiphyseal plate group,scaffold group and control,the shorten deformity were respectively 3.24 ± 0.58,6.32 ± 0.37 and 8.47 ± 0.51,while the varus deformity for them were 4.89 ± 1.27,10.67 ± 1.58 and 18.44 ± 2.65.The difference among them was statistically significant (P =0.0001 ).Conclusions The engineered epiphyseal plate is a better and promising biomaterial to treat epiphyseal plate injury.