中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2008年
5期
278-280
,共3页
耿华欧%章锦才%胡彬%王京滨
耿華歐%章錦纔%鬍彬%王京濱
경화구%장금재%호빈%왕경빈
四氯二苯并二(噁)英%环境暴露%牙槽骨质丢失%骨组织形态计量学
四氯二苯併二(噁)英%環境暴露%牙槽骨質丟失%骨組織形態計量學
사록이분병이(오)영%배경폭로%아조골질주실%골조직형태계량학
Tetrachlorodibenzodioxin%Environmental exposure%Alveolar bone loss%Bone histomorphometry
目的 研究鼠哺乳期二(噁)英类物质2,3,7,8-四氯二苯对二(噁)英暴露对大鼠仔鼠牙槽骨生长发育的影响.方法 60天龄2,3,7,8-四氯二苯对二(噁)英暴露组和对照组仔大鼠被处死,将其带有磨牙的上颌骨行树脂包埋,制备硬组织磨片并在荧光显微镜下观察,对两实验组牙槽骨的组织形态、荧光标记情况及组织形态计量学参数进行比较.结果 与对照组相比,二(噁)英暴露组牙槽骨骨小梁结构较为疏松,荧光标记较紊乱.实验组与对照组骨小梁宽度分别为(52.5±5.2)μm及(59.4±6.6)μm,二者差异有统计学意义(P<0.05);骨小梁数目分别为(3.27±0.23)mm-1及(3.75±0.29)mm-1,差异有统计学意义(P<0.01);骨小梁分离度分别为(217.3±37.6)μm及(177.6±33.8)μm,差异有统计学意义(P<0.05);矿化沉积率分别为(0.68±0.08)μm/d及(0.95±0.12)μm/d,差异亦有统计学意义(P<0.05).结论 二(噁)英类物质哺乳期暴露显著降低了大鼠仔鼠牙槽骨的质、量,并影响其空间构型.
目的 研究鼠哺乳期二(噁)英類物質2,3,7,8-四氯二苯對二(噁)英暴露對大鼠仔鼠牙槽骨生長髮育的影響.方法 60天齡2,3,7,8-四氯二苯對二(噁)英暴露組和對照組仔大鼠被處死,將其帶有磨牙的上頜骨行樹脂包埋,製備硬組織磨片併在熒光顯微鏡下觀察,對兩實驗組牙槽骨的組織形態、熒光標記情況及組織形態計量學參數進行比較.結果 與對照組相比,二(噁)英暴露組牙槽骨骨小樑結構較為疏鬆,熒光標記較紊亂.實驗組與對照組骨小樑寬度分彆為(52.5±5.2)μm及(59.4±6.6)μm,二者差異有統計學意義(P<0.05);骨小樑數目分彆為(3.27±0.23)mm-1及(3.75±0.29)mm-1,差異有統計學意義(P<0.01);骨小樑分離度分彆為(217.3±37.6)μm及(177.6±33.8)μm,差異有統計學意義(P<0.05);礦化沉積率分彆為(0.68±0.08)μm/d及(0.95±0.12)μm/d,差異亦有統計學意義(P<0.05).結論 二(噁)英類物質哺乳期暴露顯著降低瞭大鼠仔鼠牙槽骨的質、量,併影響其空間構型.
목적 연구서포유기이(오)영류물질2,3,7,8-사록이분대이(오)영폭로대대서자서아조골생장발육적영향.방법 60천령2,3,7,8-사록이분대이(오)영폭로조화대조조자대서피처사,장기대유마아적상합골행수지포매,제비경조직마편병재형광현미경하관찰,대량실험조아조골적조직형태、형광표기정황급조직형태계량학삼수진행비교.결과 여대조조상비,이(오)영폭로조아조골골소량결구교위소송,형광표기교문란.실험조여대조조골소량관도분별위(52.5±5.2)μm급(59.4±6.6)μm,이자차이유통계학의의(P<0.05);골소량수목분별위(3.27±0.23)mm-1급(3.75±0.29)mm-1,차이유통계학의의(P<0.01);골소량분리도분별위(217.3±37.6)μm급(177.6±33.8)μm,차이유통계학의의(P<0.05);광화침적솔분별위(0.68±0.08)μm/d급(0.95±0.12)μm/d,차이역유통계학의의(P<0.05).결론 이(오)영류물질포유기폭로현저강저료대서자서아조골적질、량,병영향기공간구형.
Objective To study the influence of lactational dioxin exposure(2,3,7,8-tetrachlorodibenzo-p-dixon,TCDD)on development of alveolar bone in SD rat offspring.Methods The rats of TCDD exposure group and control group were sacrificed and the alveolar bone with molars of PD60 rats in the two groups were embedded in resin.The sections were observed by fluorescent microscope.The alveolar bone formation was evaluated by histological examination,tetracycline fluorescence marker and quantitative histomorphometry.The indices of quantitative histomorphometry were compaired.Resuits The trabecular structure of abeolar bone was looser in TCDD exposure group than in the control group.The tetracycline fluorescence markers were more disorganized in TCDD group.The indices of quantitative histomorphometry of alveolar bone between two groups showed significantly difierent. Conclusions Lactational 2,3,7,8-TCDD exposure decreased the quality and quantity of alveolar bone in SD rat offspring.It is suggested that dioxins exposure may interrupt the spatial configuration.
