CAJ | 학술논문

目的观察硬膜外注射不同剂量的酒石酸布托啡诺(布托啡诺)对大鼠血清髓鞘碱性蛋白(myelin basic protein,MBP)和脊髓形态学的影响.方法雄性SD大鼠,体重180 g～210 g,于L1-2处行硬膜外置入PE-530导管,3d后,取无运动障碍的大鼠32只,随机数字表法分为4组(每组8例):生理盐水组(NS组)、布托啡诺组(B1组；B2组；B3组).NS组硬膜外注射生理盐水30μl,B1、B2、B3组分别硬膜外注射布托啡诺60、120、240 μg(均生理盐水稀释至30μl).置管成功后1 d～5d给药,1次/天.动物在给药前(T0)、末次给药后6(T1)、24(T2)、72 h(T3)采血,检测血清MBP.末次给药后72 h(T3),取脊髓腰膨大,在光镜和电镜下观察其神经形态学和细胞凋亡的变化.结果各组血清MBP差异无统计学意义(P＞0.05)；神经细胞内的尼氏体与NS组(39.2±3.4)和B1组(35.1±2.7)相比,B2组(21.2±2.6)和B3组(25.3±3.1)中数量减少(P＜0.05)；Bcl-2蛋白的表达与NS组(10.6±1.6)和B1组(13.4±2.1)相比,B2组(36.3±7.2)和B3组(48.2±8.5)明显增加(P＜0.05)；Bax蛋白的表达与NS组(8.6±2.5)和B1组(10.2±3.1)相比,B2组(37.9±7.6)和B3组(45.2±8.3)明显增加(P＜0.05)；B2组(15.7±2.6)和B3组(18.8±3.2)与NS组(6.1±2.5)和B1组(9.7±2.9)相比,可见凋亡细胞明显增多(P＜0.05)；电镜下可见髓鞘结构破坏,但未出现脱髓鞘现象.结论布托啡诺60 μg连续给药对大鼠脊髓形态功能无影响,但布托啡诺120 μg和布托啡诺240 μg连续给药会损害大鼠脊髓形态功能.
목적 관찰경막외주사불동제량적주석산포탁배낙(포탁배낙)대대서혈청수초감성단백(myelin basic protein,MBP)화척수형태학적영향.방법 웅성SD대서,체중180 g～210 g,우L1-2처행경막외치입PE-530도관,3d후,취무운동장애적대서32지,수궤수자표법분위4조(매조8례):생리염수조(NS조)、포탁배낙조(B1조；B2조；B3조).NS조경막외주사생리염수30μl,B1、B2、B3조분별경막외주사포탁배낙60、120、240 μg(균생리염수희석지30μl).치관성공후1 d～5d급약,1차/천.동물재급약전(T0)、말차급약후6(T1)、24(T2)、72 h(T3)채혈,검측혈청MBP.말차급약후72 h(T3),취척수요팽대,재광경화전경하관찰기신경형태학화세포조망적변화.결과 각조혈청MBP차이무통계학의의(P＞0.05)；신경세포내적니씨체여NS조(39.2±3.4)화B1조(35.1±2.7)상비,B2조(21.2±2.6)화B3조(25.3±3.1)중수량감소(P＜0.05)；Bcl-2단백적표체여NS조(10.6±1.6)화B1조(13.4±2.1)상비,B2조(36.3±7.2)화B3조(48.2±8.5)명현증가(P＜0.05)；Bax단백적표체여NS조(8.6±2.5)화B1조(10.2±3.1)상비,B2조(37.9±7.6)화B3조(45.2±8.3)명현증가(P＜0.05)；B2조(15.7±2.6)화B3조(18.8±3.2)여NS조(6.1±2.5)화B1조(9.7±2.9)상비,가견조망세포명현증다(P＜0.05)；전경하가견수초결구파배,단미출현탈수초현상.결론 포탁배낙60 μg련속급약대대서척수형태공능무영향,단포탁배낙120 μg화포탁배낙240 μg련속급약회손해대서척수형태공능.
Objective To observe the effect of epidural injection with different dose of tartrate-butorphanol(butorphanol)on the rats' serum myelin basic protein(MBP)and spinal cord morphology.Methods A PE-530 catheter was inserted into the epidural space of each Sprague-Dawley rat(male,weighting 180 g-210 g)at L1-2 level,and three days later,a total of 32 rats without any motor dysfunction were divided into 4 groups by random number table method as follow:saline group(group NS,n=8)and butorphanol group(B1,n=8;B2,n=8;B3,n=8).Rats in group NS were epidurally injected NS 30 μl each,and rats in group B1,B2 and B3 were respectively epidurally injected butorphanol 60,120 μg and 240 μg(all diluted to 30 μl with NS)1 time per day 5 d,the serum MBP of rats were measured before injection(T0),6(T1),24(T2)and 72 h(T3)after the last injection.At T3,the lumbar enlargement of the spinal cord was taken for detecting the morphology and apoptosis with optical microscope and electron microscope.Results There was no significant difference between each group in content of the serum MBP(P＜0.05).In group B2(21.2±2.6)and B3(25.3±3.1),compared with group NS(39.2±3.4)and B1(35.1±2.7),the number of nissl body in the neurons were decrease(P＜0.05).In group B2(36.3±7.2)and B3(48.2±8.5),compared with group NS(10.6±1.6)and B1(13.4±2.1),the expression of the Bcl-2 decreased obviously(P＜0.05).In group B2(37.9 ±7.6)and B3(45.2 ±8.3),compared with group NS(8.6 ±2.5)and B1(10.2 ±3.1),the expression of the Bax increased obviously(P＜0.05).In group B2(15.7±2.6)and B3(18.8±3.2),compared with group NS(6.1 ±2.5)and B1(9.7±2.9),the number of apoptosis neurons increased obviously(P＜0.05).In the electron microscope,the structure of myelins was destroyed,but not demyelinated.Conclusion Repeated epidural injection of butorphanol 60 pg has no influence on spinal cord morphology of rats,while injection of 120 μg and 240 μg can impair the spinal cord morphology.