CAJ | 학술논문

目的探讨糖尿病足截肢患者股神经雪旺氏细胞中小窝蛋白1的表达及意义.方法 40例糖尿病足截肢患者根据2型糖尿病病程分为A(＜6年),B(6～10年),C(＞10年)3组.HE、Weil氏染色观察股神经病理学改变,银染法进行轴突染色并计数股神经纤维密度.应用免疫组化染色检测雪旺氏细胞中小窝蛋白1的表达.结果 3组股神经均存在明显的病理改变,随着病程延长病变加重.3组间股神经纤维密度差异有统计学意义(P＜0.05),股神经纤维密度与HbA1C病程呈负相关(r分别为-0.792和-0.592,均P＜0.01),与小窝蛋白1呈正相关(r=0.721,P＜0.01).3组股神经雪旺氏细胞中均有小窝蛋白1的表达,差异有统计学意义(P＜0.01),小窝蛋白1与HbA1C、病程呈负相关(r分别为-0.762和-0.532,均P＜0.01),与神经纤维密度呈正相关(r=0.721,P＜0.01),小窝蛋白1与HbA1C的偏相关系数r=-0.505(P＜0.01).结论因足病截肢的患者中,小窝蛋白1在糖尿病周围神经病变及糖尿病足形成过程中可能起重要作用.
목적 탐토당뇨병족절지환자고신경설왕씨세포중소와단백1적표체급의의.방법 40례당뇨병족절지환자근거2형당뇨병병정분위A(＜6년),B(6～10년),C(＞10년)3조.HE、Weil씨염색관찰고신경병이학개변,은염법진행축돌염색병계수고신경섬유밀도.응용면역조화염색검측설왕씨세포중소와단백1적표체.결과 3조고신경균존재명현적병리개변,수착병정연장병변가중.3조간고신경섬유밀도차이유통계학의의(P＜0.05),고신경섬유밀도여HbA1C병정정부상관(r분별위-0.792화-0.592,균P＜0.01),여소와단백1정정상관(r=0.721,P＜0.01).3조고신경설왕씨세포중균유소와단백1적표체,차이유통계학의의(P＜0.01),소와단백1여HbA1C、병정정부상관(r분별위-0.762화-0.532,균P＜0.01),여신경섬유밀도정정상관(r=0.721,P＜0.01),소와단백1여HbA1C적편상관계수r=-0.505(P＜0.01).결론 인족병절지적환자중,소와단백1재당뇨병주위신경병변급당뇨병족형성과정중가능기중요작용.
Objective To investigate the expression and significance of caveolin-1 in femoral nerve of diabetic patients with foot amputation. Methods Forty patients with foot amputation were assigned to 3 groups according to their duration of type 2 diabetes: group A ( ＜6 years＝, group B (6-10 years), and group C ( ＞10 years). Hematoxylin and eosin (HE) stain and Weil's stain were used to examine the femoral nerve. Silver staining was used to observe the axons and to count the nerve fiber density. The expression of caveolin-1 in Schwann cells of femoral nerve was tested by immunohistochemisty. Results There were evident progressive pathological changes in femoral nerve in the 3 groups. The variance of nerve fiber density in the 3 groups reached statistical significance ( P＜0. 05 ＝, the nerve fiber density showed negative correlation with HbA1C( r =-0. 792, P＜0. 01 ＝ and duration ( r=-0.592, P＜0. 01 ＝. The expression of caveolin-1 in Schwann cells of femoral nerve was positive in all the 3 groups and the variance with statistical significance (P＜0. 01 ), it was negatively correlated with HbA1C (r=-0. 762, P＜0. 01 )and duration (r=-0. 532, P＜0. 01 ), and it was positively correlated with nerve fiber density (r=0. 721, P＜0.01 ), the partial correlation coefficient of caveolin-1 and HbA1Cwas-0. 505 ( P＜0. 01 ).Conclusion In patients with diabetic foot amputation, caveolin-1 may play a role in the development of diabetic peripheral neuropathy and diabetic foot.