中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2008年
19期
1354-1358
,共5页
胡益民%杨晓苏%张长应%肖波
鬍益民%楊曉囌%張長應%肖波
호익민%양효소%장장응%초파
偏头痛%模型,动物%硝酸甘油%三叉颈复合体%P型Na+-K+-交换ATP酶
偏頭痛%模型,動物%硝痠甘油%三扠頸複閤體%P型Na+-K+-交換ATP酶
편두통%모형,동물%초산감유%삼차경복합체%P형Na+-K+-교환ATP매
Migraine%Models,animal%Glyceryl trinitrate%Trigeminocervical complex%Ptype Na + -K + -exchanging ATPase
目的 利用硝酸甘油实验性偏头痛模型探讨P型Na+-K+-ATP酶在偏头痛发病机制中的作用.方法 20只SD大鼠(雌雄各半)随机分为生理盐水对照组和模型组.模型组按TassorelliCristina法复制偏头痛大鼠模型(每周1次,连续4次),第4次造模后分别取三叉神经节及三叉颈复合体、大脑额叶组织;RT-PCR及Western印迹法分别测定P型Na+-K+-ATP酶的mRNA和蛋白的表达量,免疫沉淀法测定其活性,同时用Fluo-3/AM荧光法测定细胞内钙离子浓度.结果 模型组大鼠三叉神经节及三叉颈复合体(0.72±0.05,0.59±0.05,5.21±0.51)及大脑额叶组织(0.70±0.05,0.60±0.05,3.61±0.49)的P型Na泵mRNA及蛋白表达和活性均明显低于生理盐水对照组(0.83±0.10,0.67±0.06,6.53±0.73;0.81±0.08,0.71±0.09,6.61±0.73),差异有统计学意义;模型组大鼠三叉神经节及三叉颈复合体(211 182±12 973)及大脑额叶组织(186 511±18 297)的细胞内钙离子浓度均明显高于生理盐水对照组(135 243±18 105,143 289±25 175),差异有统计学意义.结论 P型Na+-K+-ATP酶可能通过其表达量及活性的变化参与了偏头痛的发生发展过程.
目的 利用硝痠甘油實驗性偏頭痛模型探討P型Na+-K+-ATP酶在偏頭痛髮病機製中的作用.方法 20隻SD大鼠(雌雄各半)隨機分為生理鹽水對照組和模型組.模型組按TassorelliCristina法複製偏頭痛大鼠模型(每週1次,連續4次),第4次造模後分彆取三扠神經節及三扠頸複閤體、大腦額葉組織;RT-PCR及Western印跡法分彆測定P型Na+-K+-ATP酶的mRNA和蛋白的錶達量,免疫沉澱法測定其活性,同時用Fluo-3/AM熒光法測定細胞內鈣離子濃度.結果 模型組大鼠三扠神經節及三扠頸複閤體(0.72±0.05,0.59±0.05,5.21±0.51)及大腦額葉組織(0.70±0.05,0.60±0.05,3.61±0.49)的P型Na泵mRNA及蛋白錶達和活性均明顯低于生理鹽水對照組(0.83±0.10,0.67±0.06,6.53±0.73;0.81±0.08,0.71±0.09,6.61±0.73),差異有統計學意義;模型組大鼠三扠神經節及三扠頸複閤體(211 182±12 973)及大腦額葉組織(186 511±18 297)的細胞內鈣離子濃度均明顯高于生理鹽水對照組(135 243±18 105,143 289±25 175),差異有統計學意義.結論 P型Na+-K+-ATP酶可能通過其錶達量及活性的變化參與瞭偏頭痛的髮生髮展過程.
목적 이용초산감유실험성편두통모형탐토P형Na+-K+-ATP매재편두통발병궤제중적작용.방법 20지SD대서(자웅각반)수궤분위생리염수대조조화모형조.모형조안TassorelliCristina법복제편두통대서모형(매주1차,련속4차),제4차조모후분별취삼차신경절급삼차경복합체、대뇌액협조직;RT-PCR급Western인적법분별측정P형Na+-K+-ATP매적mRNA화단백적표체량,면역침정법측정기활성,동시용Fluo-3/AM형광법측정세포내개리자농도.결과 모형조대서삼차신경절급삼차경복합체(0.72±0.05,0.59±0.05,5.21±0.51)급대뇌액협조직(0.70±0.05,0.60±0.05,3.61±0.49)적P형Na빙mRNA급단백표체화활성균명현저우생리염수대조조(0.83±0.10,0.67±0.06,6.53±0.73;0.81±0.08,0.71±0.09,6.61±0.73),차이유통계학의의;모형조대서삼차신경절급삼차경복합체(211 182±12 973)급대뇌액협조직(186 511±18 297)적세포내개리자농도균명현고우생리염수대조조(135 243±18 105,143 289±25 175),차이유통계학의의.결론 P형Na+-K+-ATP매가능통과기표체량급활성적변화삼여료편두통적발생발전과정.
Objective To investigate the role of P-type Na+ -K+ -ATPase in the mechanism of migraine. Methods nitroglycerin induced migraine Twenty Sprague-Dawley rats, 10 male and 10 female,were randomly divided into 2 groups: model group, undergoing subcutaneous injection of nitroglycerin 10 mg/kg once a week for 4 weeks so as to establish migraine model, and control group, undergoing subcutaneous injection of normal saline. Then the rats were killed with their trigeminal ganglia,trigeminocervical complex, and cortex of frontal lobe taken out. RT-PCR and Western-blotting were used to detect the mRNA and protein expression of P-type Na+ -K+ -ATPase. Immuno-precipitation assay was conducted to observe the Na + -K + -ATPase activity. The concentration of intracellular Ca2 + was investigated by Fluo-3/AM fluorometric method. Results The mRNA and protein expression levels and activity of P-type Na + -K + -ATPase in the trigeminal ganglion and trigeminocervical complex of the model group were 0. 72 ±0. 05,0. 59 ± 0. 05 ,and 5.21 ± 0. 51 respectively, and the mRNA and protein expression levels and activity of P-type Na + -K + -ATPase in the cortex of frontal lobe of the model group were 0. 70 ± 0. 05,0. 60 ± 0. 05,and 3.61 ± 0. 49 respectively, all significantly lower than those of the control group (0. 83 ± 0. 10,0. 67 ±0.06,6.53 ±0.73,0.81 ±0.08,0.71 ± 0.09, and 6.61 ±0.73 respectively, all P ＜0.05). The concentration of intracellular Ca2+ in trigeminal ganglion and trigeminocervical complex and the concentration of Ca2+ in the cortex of frontal lobe of the model group were 211 182 ± 12 973 and 186 511 ± 18 297respectively, both significantly higher than those of the control group ( 135 243 ± 18 105 and 143 289 ±25 175 respectively, both P ＜ O. 01 ). Conclusion P-type Na + -K + -ATPase may infect the pathogenesis of migraine by its expression and activity.
