中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2011年
6期
573-576
,共4页
王亚玲%王洪格%陆岩%陈晓隆
王亞玲%王洪格%陸巖%陳曉隆
왕아령%왕홍격%륙암%진효륭
眼内炎/药物疗法%万古霉素/投药和剂量%药代动力学%疾病模型%动物
眼內炎/藥物療法%萬古黴素/投藥和劑量%藥代動力學%疾病模型%動物
안내염/약물요법%만고매소/투약화제량%약대동역학%질병모형%동물
Endophthalmitis/ drug therapy%Vancomycin/administration & dosage%Pharmacokinetics%Disease models,animal
目的 观察不同病理状态兔眼玻璃体内万古霉素的浓度和药代动力学参数.方法 健康成年白化兔81只,随机分为有晶状体细菌性眼内炎组(A组)、晶状体摘除手术后细菌性眼内炎组(B组)及晶状体摘除手术后眼内炎并行玻璃体切割手术组(C组),每组27只.所有兔右眼玻璃体腔注射浓度为10 mg/ml的万古霉素溶液1 ml.注射后0.5、2.0、4.0、6.0、12.0、24.0、48.0、72.0、84.0 h,每组注气处死3只兔,摘取眼球,收集玻璃体样品.采用高效液相色谱法(HPLC-UV)检测各组兔眼玻璃体内万古霉素浓度.应用3p 97药代动力学软件拟合并计算3组玻璃体内万古霉素的药-时曲线下面积(AUC)、清除率(CL)、半衰期(t1/2)及峰值浓度(Cmax)等药代动力学参数.结果 玻璃体腔注射万古霉素后各时间点,A组万古霉素浓度均高于治疗浓度.玻璃体腔注射后0.5、2.0、4.0、6.0、12.0h,B、C组万古霉素均维持较高浓度；玻璃体腔注射后24、48 h,浓度下降较快；玻璃体腔注射后72 h,浓度低于最低抑菌浓度.注射后不同时间点3组万古霉素浓度比较,A组与B、C组间差异均有统计学意义(P＜0.05)；B、C组间差异无统计学意义(P＞0.05).A、B、C组万古霉素AUC分别为15 790.61、7643.94、7443.44 μg/(ml·h),CL分别为0.063、0.131、0.134ml/h,t1/2分别为13.49、7.15、6.93 h,Cmax分别为711.56、648.45、667.74μg/ml.A组与B、C组比较,万古霉素AUC较大(t=4.963,5.097；P＜0.01),CL较低(t=2.963,3.097;P＜0.05),t1/2较长(t=3.315,3.481; P＜0.01);但Cmax无明显差异(t=1.687,1.214；P＞0.05).结论 不同病理状态兔眼玻璃体内万古霉素的浓度及其药代动力学参数均存在一定差异.
目的 觀察不同病理狀態兔眼玻璃體內萬古黴素的濃度和藥代動力學參數.方法 健康成年白化兔81隻,隨機分為有晶狀體細菌性眼內炎組(A組)、晶狀體摘除手術後細菌性眼內炎組(B組)及晶狀體摘除手術後眼內炎併行玻璃體切割手術組(C組),每組27隻.所有兔右眼玻璃體腔註射濃度為10 mg/ml的萬古黴素溶液1 ml.註射後0.5、2.0、4.0、6.0、12.0、24.0、48.0、72.0、84.0 h,每組註氣處死3隻兔,摘取眼毬,收集玻璃體樣品.採用高效液相色譜法(HPLC-UV)檢測各組兔眼玻璃體內萬古黴素濃度.應用3p 97藥代動力學軟件擬閤併計算3組玻璃體內萬古黴素的藥-時麯線下麵積(AUC)、清除率(CL)、半衰期(t1/2)及峰值濃度(Cmax)等藥代動力學參數.結果 玻璃體腔註射萬古黴素後各時間點,A組萬古黴素濃度均高于治療濃度.玻璃體腔註射後0.5、2.0、4.0、6.0、12.0h,B、C組萬古黴素均維持較高濃度；玻璃體腔註射後24、48 h,濃度下降較快；玻璃體腔註射後72 h,濃度低于最低抑菌濃度.註射後不同時間點3組萬古黴素濃度比較,A組與B、C組間差異均有統計學意義(P＜0.05)；B、C組間差異無統計學意義(P＞0.05).A、B、C組萬古黴素AUC分彆為15 790.61、7643.94、7443.44 μg/(ml·h),CL分彆為0.063、0.131、0.134ml/h,t1/2分彆為13.49、7.15、6.93 h,Cmax分彆為711.56、648.45、667.74μg/ml.A組與B、C組比較,萬古黴素AUC較大(t=4.963,5.097；P＜0.01),CL較低(t=2.963,3.097;P＜0.05),t1/2較長(t=3.315,3.481; P＜0.01);但Cmax無明顯差異(t=1.687,1.214；P＞0.05).結論 不同病理狀態兔眼玻璃體內萬古黴素的濃度及其藥代動力學參數均存在一定差異.
목적 관찰불동병리상태토안파리체내만고매소적농도화약대동역학삼수.방법 건강성년백화토81지,수궤분위유정상체세균성안내염조(A조)、정상체적제수술후세균성안내염조(B조)급정상체적제수술후안내염병행파리체절할수술조(C조),매조27지.소유토우안파리체강주사농도위10 mg/ml적만고매소용액1 ml.주사후0.5、2.0、4.0、6.0、12.0、24.0、48.0、72.0、84.0 h,매조주기처사3지토,적취안구,수집파리체양품.채용고효액상색보법(HPLC-UV)검측각조토안파리체내만고매소농도.응용3p 97약대동역학연건의합병계산3조파리체내만고매소적약-시곡선하면적(AUC)、청제솔(CL)、반쇠기(t1/2)급봉치농도(Cmax)등약대동역학삼수.결과 파리체강주사만고매소후각시간점,A조만고매소농도균고우치료농도.파리체강주사후0.5、2.0、4.0、6.0、12.0h,B、C조만고매소균유지교고농도；파리체강주사후24、48 h,농도하강교쾌；파리체강주사후72 h,농도저우최저억균농도.주사후불동시간점3조만고매소농도비교,A조여B、C조간차이균유통계학의의(P＜0.05)；B、C조간차이무통계학의의(P＞0.05).A、B、C조만고매소AUC분별위15 790.61、7643.94、7443.44 μg/(ml·h),CL분별위0.063、0.131、0.134ml/h,t1/2분별위13.49、7.15、6.93 h,Cmax분별위711.56、648.45、667.74μg/ml.A조여B、C조비교,만고매소AUC교대(t=4.963,5.097；P＜0.01),CL교저(t=2.963,3.097;P＜0.05),t1/2교장(t=3.315,3.481; P＜0.01);단Cmax무명현차이(t=1.687,1.214；P＞0.05).결론 불동병리상태토안파리체내만고매소적농도급기약대동역학삼수균존재일정차이.
Objective To observe the concentration of vancomycin and the changes of pharmacokinetic parameters in rabbit vitreous with endophthalmitis in different pathological conditions.Methods Eighty-one adult healthy rabbits were randomly divided into endophthalmitis with lens group (group A),aphakic endophthalmitis group (group B),aphakic endophthalmitis and vitrectomy group (group C),27 rabbits in each group.The right eyes of all rabbits received intravitreal injection of 1 ml (10 mg/ml) vancomycin.Three rabbits from each group were sacrificed at 0.5,2.0,4.0,6.0,12.0,24.0,48.0,72.0,84.0 hours after the injection.The eyes were harvested to collect the vitreous.The vitreous concentrations of vancomycin in all the groups were detected by high performance liquid chromatography (HPLC-UV).The pharmacokinetic parameters including the area under the curve (AUC) of the concentration-time graph,clearance rate (CL),half-life period (t1/2) and peak concentration (Cmax) were calculated by 3p 97 pharmacokinetic software.Results The concentrations of vancomycin in the group A were always higher than the therapeutic drug levels after injection.In the group B and C,the concentrations of vancomycin remained significantly high at 0.5,2.0,4.0,6.0 and 12.0 hours after injection,decreased quickly at 24 and 48 hours after injection,below the minimal inhibitory concentrations at 72 hours after injection.The differences were statistically significant among group A,B and C (t =4.968,5.232; P＜0.05),but not statistically significant between group B and C (t=1.279,P＞0.05).The AUC were 15 790.61,7643.94,7443.44 μg/(ml · h),CL were 0.063,0.131,0.134 ml/h,t1/2 were 13.49,7.15,6.93 hours and Cmax were 711.56,648.45,667.74 μg/ml in the group A,B and C,respectively.In the group A,the CL was lower (t=2.963,3.097 ; P＜0.05 ) and t1/2 was longer (t=3.315,3.481 ; P＜0.01 ) than those in the group B and C,but there was no significant difference on Cmax (t =1.687,1.214;P＞0.05).Conclusion The pharmacokinetic parameters of vancomycin in rabbit vitreous with endophthalmitis varied between different pathological conditions.
