CAJ | 학술논문

目的研究桂西地区对克拉霉素耐药的Hp基因分型.方法于2007年3-10月在右江民族医学院附属医院胃镜室采集就诊患者胃黏膜标本,共采集患有胃炎、消化性溃疡的患者胃黏膜标本247份,分离培养获得126株Hp,E-teat进行药敏实验,REP-PCR方法进行基因分型,并运用NTsys_2软件进行聚类分析,同时收集携带耐药菌株病例的临床资料.结果桂西地区对克拉霉素耐药的26株Hp按照相似性78%被分成6类基因型,分别是Ⅰ-Ⅵ类,每类分别有2、11、1、8、3、1株耐药性Hp;其中Ⅱ类菌株均来自胃溃疡,而且大多数来自壮族患者;Ⅳ类菌株均来自胃炎.结论 REP-PCR可以把对克拉霉素耐药的Hp分成6大类基因型,Hp感染患者的疾病类型、民族、家族胃病史等可能与基因型有关.
목적 연구계서지구대극랍매소내약적Hp기인분형.방법 우2007년3-10월재우강민족의학원부속의원위경실채집취진환자위점막표본,공채집환유위염、소화성궤양적환자위점막표본247빈,분리배양획득126주Hp,E-teat진행약민실험,REP-PCR방법진행기인분형,병운용NTsys_2연건진행취류분석,동시수집휴대내약균주병례적림상자료.결과 계서지구대극랍매소내약적26주Hp안조상사성78%피분성6류기인형,분별시Ⅰ-Ⅵ류,매류분별유2、11、1、8、3、1주내약성Hp;기중Ⅱ류균주균래자위궤양,이차대다수래자장족환자;Ⅳ류균주균래자위염.결론 REP-PCR가이파대극랍매소내약적Hp분성6대류기인형,Hp감염환자적질병류형、민족、가족위병사등가능여기인형유관.
Objective To study the genotyping of clarithromycin-resistant Helicobacter pylori(Hp) isolates.Methods From March 2007 to October 2007,247 gastric mucosa specimens were collected by the endoscopy from the patients with peptic ulcer or gastritis at the affiliated hospital of Youjiang Medical College for Nationalities.A total of 126 Hp strains were isolated.Resistance to clarithromycin in Hp was determined by E-test.All of the resistant isolates were genotyped with REP-PCR and further clustered with NTsys_2 software.The clinical data was collected for these patients with clarithromycin-resistant Hp.Results The 26 clarithromycin-resistant isolates from the west of Guangxi were divided into six genotypea including Group Ⅰ,Group Ⅱ,Group Ⅲ,Group Ⅲ,Group Ⅳ,Group Ⅴ and Group Ⅵ according to the homology of 78%.Every group have 2,11,1,8,3,1 strains Hp,respectively.Strains of group Ⅱ were isolated from patients with peptic ulcer and most of them from Chuang patients.All strains of group Ⅳ were isolated from patients with gastritis.Conclusions The clarithromycin-resistant isolates were divided into six groups by REP-PCR Disease type.nationality of patients and family history of stomach diseases were associated with the genotypes.