CAJ | 학술논문

抗除草剂作物占全球转基因作物的75%以上,并且这类作物还有不断增加的趋势.首次建立并优化了对4类转基因作物(大豆(Glycincmax)、玉米(Zeamays)、棉花(Gossypiumhirsutnm)和油菜(Brassica campestrisvar.oleifera))的5种外源抗除草剂基因(bar、pat、cp4-epspsl、cp4-sps2和gox)的PCR检测方法,并在实践中应用.结果表明,本方法灵敏特异、结果准确可靠,可对目前批准的所有抗除草剂转基因产品进行定性检测.
항제초제작물점전구전기인작물적75%이상,병차저류작물환유불단증가적추세.수차건립병우화료대4류전기인작물(대두(Glycincmax)、옥미(Zeamays)、면화(Gossypiumhirsutnm)화유채(Brassica campestrisvar.oleifera))적5충외원항제초제기인(bar、pat、cp4-epspsl、cp4-sps2화gox)적PCR검측방법,병재실천중응용.결과표명,본방법령민특이、결과준학가고,가대목전비준적소유항제초제전기인산품진행정성검측.
The GMO (genetically modified organisms) containing anti-herbicide genes account for more than 75% of all GMO and the proportion is increasing. The 5 kinds of the anti-herbicide genes (bar, pat, cp4-epspsl, cp4-epsps2 and gox) were examined by PCR in 4 kinds of genetically modified crops (soybean (Glycine max), maize (Zea mays), cotton (Gossypinm hirsutnm) and rape (Brassica campestris var. oleifera)). Results indicated that the method found was sensitive, specific and credible, and all genetically modified crops containing the anti-herbicide genes could be analyzed by this method.