CAJ | 학술논문

本文通过对剪形臂尾轮虫、矩形臂尾轮虫、十指臂尾轮虫、红臂尾轮虫、角突臂尾轮虫、双棘臂尾轮虫、裂足臂尾轮虫和萼花臂尾轮虫等八种臂尾轮虫rDNA ITS 1序列分析,并以西氏晶囊轮虫为外群,使用PAUP和贝叶斯软件分别构建臂尾轮属轮虫系统发生树(MP树、NJ树、ML树和贝叶斯树),以探讨臂尾轮属的系统发生关系,并解决其中的一些分类问题.结果表明:本研究所涉及的轮虫rDNA ITS 1平均序列差异百分比较高,为29%;海水和淡水臂尾轮虫被明显分为不同的进化枝;除双棘臂尾轮虫外,在淡水臂尾轮虫中具有三对前棘刺且营附着生活的种类与前棘刺少于三对且营浮游生活的种类聚在不同支系中,这与以形态特征为主所进行的系统发生研究结果基本一致;所有的系统树均支持将十指臂尾轮虫作为一个独立的支系分离出来,裂足臂尾轮虫应归入臂尾轮属[动物学报52(6):1067-1074,2006].
본문통과대전형비미륜충、구형비미륜충、십지비미륜충、홍비미륜충、각돌비미륜충、쌍극비미륜충、렬족비미륜충화악화비미륜충등팔충비미륜충rDNA ITS 1서렬분석,병이서씨정낭륜충위외군,사용PAUP화패협사연건분별구건비미륜속륜충계통발생수(MP수、NJ수、ML수화패협사수),이탐토비미륜속적계통발생관계,병해결기중적일사분류문제.결과표명:본연구소섭급적륜충rDNA ITS 1평균서렬차이백분비교고,위29%;해수화담수비미륜충피명현분위불동적진화지;제쌍극비미륜충외,재담수비미륜충중구유삼대전극자차영부착생활적충류여전극자소우삼대차영부유생활적충류취재불동지계중,저여이형태특정위주소진행적계통발생연구결과기본일치;소유적계통수균지지장십지비미륜충작위일개독립적지계분리출래,렬족비미륜충응귀입비미륜속[동물학보52(6):1067-1074,2006].
The rDNA ITS 1 gene sequences of eight Brachionus species including B. forficula, B. leydigi, B. patulus,B . rubens, B . angularis , B . bidentata , B . diversicornis and B . calyci florus were analyzed, and using Asplanchna sieboldi as an outgroup, neighbor-joining ( NJ ), maximum-parsimony ( MP), maximum-likelihood (ML) and Bayesian methods were used to reveal their phylogenetic relationships and resolve some taxonomic questions. The average percentage of divergence for rDNA ITS 1 gene sequences in 31 samples was 29%. The marine and the freshwater Brachionus species were clearly divided into different clades. Among the freshwater Brachionus rotifers, with the exception of B. bidentata, species having three pairs of anterior spines and being littoral, and those having less than three pairs of anterior spines and being planktonic were grouped in different clades. This was consistent with their morphological taxonomic status. All the phylogenetic trees showed that B. patulus was separated into an independent clade, and B. diversicornis belonged to the genus Brachionus [Acta Zoologica Sinica 52 (6): 1067- 1074, 2006].