CAJ | 학술논문

目的:探讨针刺治疗Cx43基因敲除鼠胶原性关节炎(Ⅱcollagen-induced arthritis,CLA)的疗效及免疫学机制.方法:以Cx43基因敲除杂合型(Cx,43+/-)和野生型(CX43+/+)小鼠为研究对象,用牛Ⅱ型胶原诱导成CLA模型.在初次免疫后第4周开始针刺治疗,连续3周.观察关节炎分数,流式细胞术检测脾淋巴细胞中Th细胞亚群含量.结果:Cx43+/-小鼠CLA发病率及关节炎分数明显低于Cx43+/+小鼠;针刺抑制CX43+/- CIA小鼠Th1细胞分泌,降低Th1/Th2比值,对Th2细胞的分泌无明显影响;而针刺对CX43+/-CIA小鼠Th细胞亚群含量无明显影响.结论:针刺对CX43+/+CIA小鼠疗效明显优于Cx43+/-CLA小鼠,提示针刺疗效与CX43基因相关,其疗效可能是通过调节Th1/Th2使之接近动态平衡而起作用的,针刺疗效与这种调节作用成正相关.
목적:탐토침자치료Cx43기인고제서효원성관절염(Ⅱcollagen-induced arthritis,CLA)적료효급면역학궤제.방법:이Cx43기인고제잡합형(Cx,43+/-)화야생형(CX43+/+)소서위연구대상,용우Ⅱ형효원유도성CLA모형.재초차면역후제4주개시침자치료,련속3주.관찰관절염분수,류식세포술검측비림파세포중Th세포아군함량.결과:Cx43+/-소서CLA발병솔급관절염분수명현저우Cx43+/+소서;침자억제CX43+/- CIA소서Th1세포분비,강저Th1/Th2비치,대Th2세포적분비무명현영향;이침자대CX43+/-CIA소서Th세포아군함량무명현영향.결론:침자대CX43+/+CIA소서료효명현우우Cx43+/-CLA소서,제시침자료효여CX43기인상관,기료효가능시통과조절Th1/Th2사지접근동태평형이기작용적,침자료효여저충조절작용성정상관.
Objective To observe the therapeutic effect of acupuncture for coilagen-induced arthritis(CIA)in Cx43 knock-out mice and its underlying immunological mechanism.Methods Heterozygote(Cx43+/-)mice(n=64)and wild-type(Cx43+/+)mice(n=46)were used in the present study.CIA model was established by intracutaneous injection of bovine collagen ll(immunonization).Acupuncture of Z(u)s(a)nl(i)(足三里ST36)was carried out from the 4th week on after the initial immunization,once a day for 3 weeks.Arthritis score and days of onset of CIA were recorded.Intracellular contents of Th subgroups of splenic lymphocytes were detected with flow cytometry.Results The incidence rate of CIA and arthritis score in Cx43+/-mice were significantly lower than those in Cx43+/+ mice(P＜0.05).In C×43+/+ mice,3 weeks after acupunc ture treatment,the score of arthritis symptoms and signs in acupuncture group was significantly lower than that in model group(P＜0.01),while in Cx43+/-mice,no significant difference was found between model group and acupuncture group in arthritis score(P＞0.05).In comparison with control group,percentages of both Th1 and Th2 and Th1/Th2 increased significantly in Cx43+/+CIA model group(P＜0.05),while corn pared with model group,the percentage of Th1,Th2 and Th1/Th2 in acupuncture group decreased evidently (P＜0.05)or moderately,showing that acupuncture can effectively suppress arthritis-induced increase of Thlin Cx43+/-mice.In Cx43+/-mice,Th1 level and Th1/Th2 of CIA model group increased considerably in comparison with control group(P＜0.05),while compared with model group,the Th1,Th2 and Th1/Th2 of acupuncture group had no significant changes(P＞0.05),indicating that the effect of acupuncture in suppressing increase of Th1 percent and Th1/Th2 was eliminated.Conclusion Acupuncture of Z(u)s(a)nl(i)(足三里ST 36)can effectively relieve CIA symptoms and signs and inhibit increase of splenic Thl in Cx43+/+mice but has no any effect on CIA score,Th1,Th2 and Th1/Th2 in Cx43++/-mice.It shows the effect of acupunc ture in improving CIA js closely related to Cx43 gene.