国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2009年
3期
220-222
,共3页
埃希氏菌属%β内酰胺酶类%抗药性%细菌%碱基序列
埃希氏菌屬%β內酰胺酶類%抗藥性%細菌%堿基序列
애희씨균속%β내선알매류%항약성%세균%감기서렬
Escherichia%Beta lactamases%Drug resistance,bacterial%Base sequence
目的 研究产超广谱β-内酰胺酶(ESBLs)大肠埃希菌对氨基糖甙类药物的耐药特性及耐药基因表达.方法 用琼脂稀释法检测庆大霉素、阿米卡星、卡那霉素、妥布霉素、奈替米星和新霉素6种药物对37株产ESBLs大肠埃希菌的最低抑菌浓度.用PCR法检测5种氨基糖甙类药物修饰酶基因,并使用DNA测序加以证实.结果 庆大霉素、阿米卡星、卡那霉素、妥布霉素和奈替米星对37株大肠埃希菌MIC50、MIC90.均大于256 mg/L,其耐药率分别为78.4%、45.9%、72.9%、83.8%和64.9%,而新霉素仍具有较高的抗菌活性.从37株菌中检出5种修饰酶基因,aac(3)-Ⅱ、aac(6′)-Ⅰ b、aac(6′)-Ⅱ、ant(2″)-Ⅰ、ant(3″)-Ⅰ阳性率分别为56.8%、27.0%、2.7%、5.4%和13:5%,未发现aac(3)-Ⅰ基因.结论 产ESBLs的大肠埃希菌对氨基糖甙类药物的耐药与修饰酶基因的表达有关.
目的 研究產超廣譜β-內酰胺酶(ESBLs)大腸埃希菌對氨基糖甙類藥物的耐藥特性及耐藥基因錶達.方法 用瓊脂稀釋法檢測慶大黴素、阿米卡星、卡那黴素、妥佈黴素、奈替米星和新黴素6種藥物對37株產ESBLs大腸埃希菌的最低抑菌濃度.用PCR法檢測5種氨基糖甙類藥物脩飾酶基因,併使用DNA測序加以證實.結果 慶大黴素、阿米卡星、卡那黴素、妥佈黴素和奈替米星對37株大腸埃希菌MIC50、MIC90.均大于256 mg/L,其耐藥率分彆為78.4%、45.9%、72.9%、83.8%和64.9%,而新黴素仍具有較高的抗菌活性.從37株菌中檢齣5種脩飾酶基因,aac(3)-Ⅱ、aac(6′)-Ⅰ b、aac(6′)-Ⅱ、ant(2″)-Ⅰ、ant(3″)-Ⅰ暘性率分彆為56.8%、27.0%、2.7%、5.4%和13:5%,未髮現aac(3)-Ⅰ基因.結論 產ESBLs的大腸埃希菌對氨基糖甙類藥物的耐藥與脩飾酶基因的錶達有關.
목적 연구산초엄보β-내선알매(ESBLs)대장애희균대안기당대류약물적내약특성급내약기인표체.방법 용경지희석법검측경대매소、아미잡성、잡나매소、타포매소、내체미성화신매소6충약물대37주산ESBLs대장애희균적최저억균농도.용PCR법검측5충안기당대류약물수식매기인,병사용DNA측서가이증실.결과 경대매소、아미잡성、잡나매소、타포매소화내체미성대37주대장애희균MIC50、MIC90.균대우256 mg/L,기내약솔분별위78.4%、45.9%、72.9%、83.8%화64.9%,이신매소잉구유교고적항균활성.종37주균중검출5충수식매기인,aac(3)-Ⅱ、aac(6′)-Ⅰ b、aac(6′)-Ⅱ、ant(2″)-Ⅰ、ant(3″)-Ⅰ양성솔분별위56.8%、27.0%、2.7%、5.4%화13:5%,미발현aac(3)-Ⅰ기인.결론 산ESBLs적대장애희균대안기당대류약물적내약여수식매기인적표체유관.
Objective To investigate the characteristics of aminoglycoside resistance of extend-ed-spectrum β-lactamases(ESBLs)-producing Escherichia coli(E, cold and expression of aminoglyco-side-modifying enzyme genes. Methods The minimal inhibitory concentrations(MICs) of gentamicin,amikacin, kanamycin, tobramycin, netilmicin and neomycin for 37 strains of ESBLs-producing E. Coli were detected by agar dilution. In addition, six aminoglycoside-modifying enzyme genes were amplified by polymersae chain reaction(PCR) and verified by DNA sequencing. Results MIC
and MIC90 of gentamicin, amikacin, kanamycin, tobramycin and netilmicin for 37 strains of ESBLs-producing E. Co-Il all excelled 256 μg/mL, the resistance rates of the above antibiotics were 78.4%, 45.9%, 72.9%,83.8%and 64.90%, respectively. However, neomycin still had powerful antibacterial activity. In ad-dition, five modifying enzyme genes, including aac(3)-Ⅱ , aac(6′)-Ⅰ b, aac(6′)-Ⅱ , ant(2″)-Ⅰ and ant(3″)- Ⅰ genes, were found in 37 isoaltes except aac(3)- Ⅰ , and their positive rates were 56.8%,27.0 %, 2.7 %, 5.4 % and 13. 5 %, respectively. Conclusion The aminoglycoside resistance of ES-BLs-producing E. Coil may be associated with the expression of aminoglycoside-modifying enzyme genes.
