CAJ | 학술논문

采用SRAP技术分析提莫菲维小麦与葡萄牙野燕麦远缘杂交后代的真实性及其特点.结果表明,22对SRAP引物中,20对引物在双亲间扩增出多态性条带,其多态性比率为73 81%.me4-em1、me3-em5、me4-em3和me3-em3 四对引物在提莫菲维小麦与葡萄牙野燕麦杂交F_3株系中扩增出双亲特异带,表明该F_3株系具有双亲的遗传物质,该F_3株系是提莫菲维小麦和葡萄牙野燕麦成功属间杂交的真实杂种后代.在F_3株系的扩增结果中部分双亲带型消失,并且提莫菲维小麦消失的带数远少于野燕麦的;同时有非父母标记新带型出现.杂种后代DNA序列的这种变化可能有利于新形成异源多倍体小麦的快速进化、遗传协调和遗传稳定性.
채용SRAP기술분석제막비유소맥여포도아야연맥원연잡교후대적진실성급기특점.결과표명,22대SRAP인물중,20대인물재쌍친간확증출다태성조대,기다태성비솔위73 81%.me4-em1、me3-em5、me4-em3화me3-em3 사대인물재제막비유소맥여포도아야연맥잡교F_3주계중확증출쌍친특이대,표명해F_3주계구유쌍친적유전물질,해F_3주계시제막비유소맥화포도아야연맥성공속간잡교적진실잡충후대.재F_3주계적확증결과중부분쌍친대형소실,병차제막비유소맥소실적대수원소우야연맥적;동시유비부모표기신대형출현.잡충후대DNA서렬적저충변화가능유리우신형성이원다배체소맥적쾌속진화、유전협조화유전은정성.
The hybrid progenies from wide cross T.timopheevi×Avena fatua L.var.Portugal were analyzed using SRAP markers to prove their truth and understand their characterizations in the present study.Among 22 primer pairs,20 yielded the polymorphic product between the both parents,and the average polymorphic ratio was 73.81%.Four primer pairs (me4-em1,me3-em5,me4-em3 and me3-em3) produced the specific markers for either parent in the F_3 line,indicating that the F_3 line contained both parental homologues of some chromosomes,and were true intergeneric hybrid origin.The F_3 line lost some parental SRAP markers,and the markers lost from T.timopheevi were far less than those from the wild oat;meanwhile,some novel SRAP markers were generated.These DNA sequence changes might contribute to the rapid evolution of the newly formed allopolyploid and allow the divergent genomes to act in harmony.