CAJ | 학술논문

[目的]研究1种新整体透明与制片技术.[方法]将酒精溶液保存的千金藤幼嫩种子和高粱新鲜的雌蕊与珠心分离出来后,用84消毒液溶液对其进行浸泡、透明,然后按照3种制片方法进行制片处理和封片.①将材料取出放在凹玻片的凹槽内,在材料表面滴上数滴84消毒液,封片后即可进行观察.②将幼嫩种子从84消毒液中取出,放入盛有清水的小称量瓶中浸泡数分钟,然后重新换上干净的清水进行浸泡.重复漂洗数次后,将幼嫩种子放入盛有栀子色素溶液的小称量瓶中浸泡染色.当幼嫩种子被染上较深颜色时,将其取出放在凹玻片的凹槽内,滴数滴清水或栀子色素溶液,封片后即可进行观察.③将材料取出放在凹玻片的凹槽内,在材料表面滴1至数滴无水乙醇和油镜油混合液(约100∶1),并将扩散到周围的白色液体用吸水纸吸去,待混合液挥发将尽,材料完全暴露在空气中时,再次滴上数滴无水乙醇和油镜油混合液.重复2～4次后,滴数滴上述混合液,封片后即可进行观察.若要制成永久制片,则在混合液挥发将尽时,在植物材料上滴数滴油镜油后封片.观察后,可长时间保存.[结果]在普通显微镜下不仅能观察到千金藤幼嫩种子中不同发育时期的球形胚和心型胚,还能观察到高粱雌蕊的特征、珠心表面的珠心突、胚囊和承珠盘;在荧光显微镜可观察到高粱株心的胚囊和承珠盘的荧光图像.[结论]使用84消毒液作为整体透明技术的透明剂,不仅能观察保存后颜色变深或新鲜的植物材料,还能简化制片过程、缩短制片时间.
[목적]연구1충신정체투명여제편기술.[방법]장주정용액보존적천금등유눈충자화고량신선적자예여주심분리출래후,용84소독액용액대기진행침포、투명,연후안조3충제편방법진행제편처리화봉편.①장재료취출방재요파편적요조내,재재료표면적상수적84소독액,봉편후즉가진행관찰.②장유눈충자종84소독액중취출,방입성유청수적소칭량병중침포수분종,연후중신환상간정적청수진행침포.중복표세수차후,장유눈충자방입성유치자색소용액적소칭량병중침포염색.당유눈충자피염상교심안색시,장기취출방재요파편적요조내,적수적청수혹치자색소용액,봉편후즉가진행관찰.③장재료취출방재요파편적요조내,재재료표면적1지수적무수을순화유경유혼합액(약100∶1),병장확산도주위적백색액체용흡수지흡거,대혼합액휘발장진,재료완전폭로재공기중시,재차적상수적무수을순화유경유혼합액.중복2～4차후,적수적상술혼합액,봉편후즉가진행관찰.약요제성영구제편,칙재혼합액휘발장진시,재식물재료상적수적유경유후봉편.관찰후,가장시간보존.[결과]재보통현미경하불부능관찰도천금등유눈충자중불동발육시기적구형배화심형배,환능관찰도고량자예적특정、주심표면적주심돌、배낭화승주반;재형광현미경가관찰도고량주심적배낭화승주반적형광도상.[결론]사용84소독액작위정체투명기술적투명제,불부능관찰보존후안색변심혹신선적식물재료,환능간화제편과정、축단제편시간.
[Objective] The research aimed to study a new whole clearing and preparation technology. [Method] After young seeds of Stephania sp. preserved in the alcohol solution, as well as the fresh pistil and nucellus of Sorghum bicolor were separated, they were soaked and cleared in the solution of 84 disinfectant. And the plant materials could be mounted by using three methods. [Result] The globular embryos and heart-shaped embryos at different stages of Stephania sp. as well as the pistil, nucellus, embryo sac, hypostase of Sorghum bicolor could be observed under a microscope. And the embryo sac and hypostase of Sorghum bicolor could be observed under a fluorescence microscope. [Conclusion] As the disinfectant of whole clearing technology, 84 disinfectant could not only observe the dark preserved or fresh plant materials, but also simplify the preparation process and shorten the preparation time.