CAJ | 학술논문

采用超声辅助提取苦瓜中的活性成分,利用分散液液微萃取（DLLME）技术对提取液进行浓缩,设计正交试验优化DLLME条件。在优化条件下,对不同产地苦瓜的超声提取液进行微萃取,萃取液进行HPLC分析,确定6个共有峰作为苦瓜的指纹峰,采用《中药色谱指纹图谱相似度评价系统》2004A版评价指纹谱图,不同产地苦瓜的HPLC指纹谱图与对照谱图（长沙苦瓜）之间的相似度均大于0.960。本研究为天然产物的鉴定和质量控制提供了一个新的途径。
채용초성보조제취고과중적활성성분,이용분산액액미췌취（DLLME）기술대제취액진행농축,설계정교시험우화DLLME조건。재우화조건하,대불동산지고과적초성제취액진행미췌취,췌취액진행HPLC분석,학정6개공유봉작위고과적지문봉,채용《중약색보지문도보상사도평개계통》2004A판평개지문보도,불동산지고과적HPLC지문보도여대조보도（장사고과）지간적상사도균대우0.960。본연구위천연산물적감정화질량공제제공료일개신적도경。
Active ingredients in Momordica charantia were extracted with ultrasonic assistance.The extracts were concentrated by dispersive liquid-liquid microextraction（DLLME） technique.Orthogonal experimental design was adopted to optimize DLLME conditions.Under optimum conditions,the extracts of Momordica charantia from different areas were microextracted,and then were analyzed by high performance liquid chromatography（HPLC）.Six common peaks were identified as fingerprint peak.The HPLC fingerprint peaks were assessed by Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine（Version 2004A）.The similarity of the fingerprint of Momordica charantia from different areas was greater than 0.960 compared with control group from Changsha.A novel approach for characterization and quality control of natural products was provided.