CAJ | 학술논문

目的观察中药活脑康对脑缺血大鼠脑组织中TGF-β1和突触素P38表达的影响.方法用线栓法制备大鼠局灶性脑缺血模型;应用SABC免疫组化染色法观察脑组织中TGF-β1和P38的表达.结果 TGF-β1和P38在正常脑神经细胞中均呈弱阳性表达,脑缺血后,TGF-β1和P38的表达量均明显增强(与正常对照组相比P<0.01,有统计学意义);中药活脑康对缺血脑组织中TGF-β1和P38的表达有上调作用(与模型组相比分别为P<0.01和P<0.001,有统计学意义).结论中药活脑康可能是通过增加TGF-β1和P38的表达减少脑细胞的凋亡,促进突触再建,增强和完善再建突触效能发挥脑保护作用.
목적 관찰중약활뇌강대뇌결혈대서뇌조직중TGF-β1화돌촉소P38표체적영향.방법 용선전법제비대서국조성뇌결혈모형;응용SABC면역조화염색법관찰뇌조직중TGF-β1화P38적표체.결과 TGF-β1화P38재정상뇌신경세포중균정약양성표체,뇌결혈후,TGF-β1화P38적표체량균명현증강(여정상대조조상비P<0.01,유통계학의의);중약활뇌강대결혈뇌조직중TGF-β1화P38적표체유상조작용(여모형조상비분별위P<0.01화P<0.001,유통계학의의).결론 중약활뇌강가능시통과증가TGF-β1화P38적표체감소뇌세포적조망,촉진돌촉재건,증강화완선재건돌촉효능발휘뇌보호작용.
Objective To observe the effects of traditional Chinese medicine HUONAOKANG on the expressionof TGF-β1 and P38 of rats with cerebral ischemic injury. Methods Focal cerebral ischemic model of cerebralmiddle artery occlusion was duplicated with nylon thread. The expression of TGF-β1 and P38 were determinedby SABC immunohistochemical method. Results No significant TGF-β1 and P38 expression was detected incontrol group. After cerebral ischemic injury, the expression of TGF-β1 and P38 were increased (comparedwith control group, P <0.01 ). HUONAOKANG increased the expression TGF-β1 and P38 obviously ( Com-pared with ischemic group, P < 0.01 and P < 0. 001, respectively). Conclusion HUONAOKANG signifi-cantly , increased the expression of TGF-β1 and P38, which might be the mechanism of protecting ischemic in-jury of brain tissue.