中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2010年
9期
944-948
,共5页
脑损伤%液压模型%大鼠%免疫组化%白介素-6%应激性溃疡
腦損傷%液壓模型%大鼠%免疫組化%白介素-6%應激性潰瘍
뇌손상%액압모형%대서%면역조화%백개소-6%응격성궤양
Brain injury%Fluid-percussion model%Rat%Immunohistochemistry%IL-6%Stress ulcer
目的 探讨下丘脑白介素-6对应激性溃疡大鼠胃黏膜血流量、胃液pH值和胃黏膜损伤的影响.方法 采用液压打击法建立大鼠颅脑外伤应激性溃疡模型.20只雄性SD大鼠在长征医院神经外科大鼠实验房饲养1周后开始实验.大鼠被随机(随机数字法)等分为对照组、致伤1 h组、致伤6h和致伤12 h组,采用免疫组化、Western blot等方法检测致伤后下丘脑性白介素-6的表达与分布.同时,检测各组大鼠胃液pH值、胃黏膜血流量、胃黏膜损伤指数,并观察胃黏膜大体及光镜下组织病理学变化.结果 白介素-6免疫反应阳性细胞广泛分布于应激大鼠下丘脑水平的神经细胞中,在室旁核(paraventricular rucleus,PVN)附近分布尤为密集.致伤后1h胃液pH值明显降低,致伤后6 h处于最低点(与1 h组比较P＜0.05).致伤后1 h即出现胃黏膜损伤,并且胃黏膜损伤指数随时间的延长而不断增加;致伤后1 h有血流量的暂时性增加,处于波峰,6 h后趋于平稳.大体及镜下病理均提示,胃黏膜呈应激性溃疡改变.结论 在液压打击致应激性溃疡的SD大鼠中,下丘脑白介素-6可通过PVN的介导激活神经内分泌代谢,引发胃黏膜损伤.
目的 探討下丘腦白介素-6對應激性潰瘍大鼠胃黏膜血流量、胃液pH值和胃黏膜損傷的影響.方法 採用液壓打擊法建立大鼠顱腦外傷應激性潰瘍模型.20隻雄性SD大鼠在長徵醫院神經外科大鼠實驗房飼養1週後開始實驗.大鼠被隨機(隨機數字法)等分為對照組、緻傷1 h組、緻傷6h和緻傷12 h組,採用免疫組化、Western blot等方法檢測緻傷後下丘腦性白介素-6的錶達與分佈.同時,檢測各組大鼠胃液pH值、胃黏膜血流量、胃黏膜損傷指數,併觀察胃黏膜大體及光鏡下組織病理學變化.結果 白介素-6免疫反應暘性細胞廣汎分佈于應激大鼠下丘腦水平的神經細胞中,在室徬覈(paraventricular rucleus,PVN)附近分佈尤為密集.緻傷後1h胃液pH值明顯降低,緻傷後6 h處于最低點(與1 h組比較P＜0.05).緻傷後1 h即齣現胃黏膜損傷,併且胃黏膜損傷指數隨時間的延長而不斷增加;緻傷後1 h有血流量的暫時性增加,處于波峰,6 h後趨于平穩.大體及鏡下病理均提示,胃黏膜呈應激性潰瘍改變.結論 在液壓打擊緻應激性潰瘍的SD大鼠中,下丘腦白介素-6可通過PVN的介導激活神經內分泌代謝,引髮胃黏膜損傷.
목적 탐토하구뇌백개소-6대응격성궤양대서위점막혈류량、위액pH치화위점막손상적영향.방법 채용액압타격법건립대서로뇌외상응격성궤양모형.20지웅성SD대서재장정의원신경외과대서실험방사양1주후개시실험.대서피수궤(수궤수자법)등분위대조조、치상1 h조、치상6h화치상12 h조,채용면역조화、Western blot등방법검측치상후하구뇌성백개소-6적표체여분포.동시,검측각조대서위액pH치、위점막혈류량、위점막손상지수,병관찰위점막대체급광경하조직병이학변화.결과 백개소-6면역반응양성세포엄범분포우응격대서하구뇌수평적신경세포중,재실방핵(paraventricular rucleus,PVN)부근분포우위밀집.치상후1h위액pH치명현강저,치상후6 h처우최저점(여1 h조비교P＜0.05).치상후1 h즉출현위점막손상,병차위점막손상지수수시간적연장이불단증가;치상후1 h유혈류량적잠시성증가,처우파봉,6 h후추우평은.대체급경하병리균제시,위점막정응격성궤양개변.결론 재액압타격치응격성궤양적SD대서중,하구뇌백개소-6가통과PVN적개도격활신경내분비대사,인발위점막손상.
Objective To clarify the role of hypothalamic IL-6 in gastric mucosal blood flow, gastric juice pH value and gastric mucosal injury. Method Model of gastric stress ulcer was established by fluid percussion to make craniocerebral trauma. Twenty male SD rats fed for one week in the experiment room were randomly(random number) divided into control group, one hour group, six hours group and 12 hours group after injury. The levels and distribution of IL-6 in hypothalamus were detected by using immunohistochemistry and Western blot. Simultaneously, gastric pH value, gastric mucosal blood flow and gastric mucosal injury index of rats in each group were measured, and the histology of gastric mucosa was observed. Results IL-6 immunoreactive cells were widely distributed in neuronal cells of hypothalamus of the stressed rats especially in the para-ventricular nucleus (PVN).One hour after injury, the pH value rapidly declined, and the lowest point appeared 6 hours later. One hour after injury, the injury of gastric mucosa was found, and the injury became worse and worse as time got longer and longer. The ulcer index (UI) was increased. One hour after injury, there was a brief increase in blood flow to the peak in gastric mucosa, and then the blood flow declined until 6 hours elapsed and got stable. The experiment prompted the gastric stress ulcer appeared. Conclusions In the SD rats with gastric stress ulcer induced by craniocerebral injury incurred by hydraulic percussion, the hypothalamic IL-6 may activate neuronendocrine metabolism mediated through the activation of PVN, inducing gastric mucosal injury.
