中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2012年
2期
114-117
,共4页
叶展超%张文斌%陈晓莉%陈江%李忠林%柯淑女
葉展超%張文斌%陳曉莉%陳江%李忠林%柯淑女
협전초%장문빈%진효리%진강%리충림%가숙녀
骨形态生成蛋白2%上颌窦底提升术%组织工程
骨形態生成蛋白2%上頜竇底提升術%組織工程
골형태생성단백2%상합두저제승술%조직공정
Bone morphogenetic protein-2%Maxillary sinus floor augmentation%Tissue engineering
目的 评价骨形态生成蛋白2( bone morphogenetic protein-2,BMP-2)基因强化骨髓间充质干细胞(bone marrow stromal cells,BMSC)复合Bio-Oss骨在上颌窦底提升术中的效果.方法 抽取12只犬髂部骨髓体外分离扩增BMSC,分别加入含BMP-2基因、绿色荧光蛋白(green fluorescent protein,GFP)基因的腺病毒载体共培养,观察转染效率,并植入裸鼠皮下观察成骨情况.将BMP-2基因强化BMSC-Bio-Oss复合骨随机植入犬一侧上颌窦底提升形成的空腔中为实验组,另一侧植入BMSC-Bio-Oss复合骨为空白对照组.分别于术后30、60、120 d行大体观察、CT检查、组织学检查和新生骨量分析.结果 裸鼠皮下可见新生骨,荧光显微镜示踪可见BMSC.犬上颌窦底提升30 d后,大体观察和CT检查示少量新骨形成,120 d后上颌窦内新骨生成致密,仅有少量Bio-Oss骨.新生骨量分析显示,与空白对照组[(19.67 ±5.73) mm2]相比,实验组新生骨量[(24.74±6.33) mm2]显著增多(P＜0.05).结论 BMP-2基因强化BMSC-Bio-Oss复合骨应用于上颌窦底提升术可获得良好的成骨效果.
目的 評價骨形態生成蛋白2( bone morphogenetic protein-2,BMP-2)基因彊化骨髓間充質榦細胞(bone marrow stromal cells,BMSC)複閤Bio-Oss骨在上頜竇底提升術中的效果.方法 抽取12隻犬髂部骨髓體外分離擴增BMSC,分彆加入含BMP-2基因、綠色熒光蛋白(green fluorescent protein,GFP)基因的腺病毒載體共培養,觀察轉染效率,併植入裸鼠皮下觀察成骨情況.將BMP-2基因彊化BMSC-Bio-Oss複閤骨隨機植入犬一側上頜竇底提升形成的空腔中為實驗組,另一側植入BMSC-Bio-Oss複閤骨為空白對照組.分彆于術後30、60、120 d行大體觀察、CT檢查、組織學檢查和新生骨量分析.結果 裸鼠皮下可見新生骨,熒光顯微鏡示蹤可見BMSC.犬上頜竇底提升30 d後,大體觀察和CT檢查示少量新骨形成,120 d後上頜竇內新骨生成緻密,僅有少量Bio-Oss骨.新生骨量分析顯示,與空白對照組[(19.67 ±5.73) mm2]相比,實驗組新生骨量[(24.74±6.33) mm2]顯著增多(P＜0.05).結論 BMP-2基因彊化BMSC-Bio-Oss複閤骨應用于上頜竇底提升術可穫得良好的成骨效果.
목적 평개골형태생성단백2( bone morphogenetic protein-2,BMP-2)기인강화골수간충질간세포(bone marrow stromal cells,BMSC)복합Bio-Oss골재상합두저제승술중적효과.방법 추취12지견가부골수체외분리확증BMSC,분별가입함BMP-2기인、록색형광단백(green fluorescent protein,GFP)기인적선병독재체공배양,관찰전염효솔,병식입라서피하관찰성골정황.장BMP-2기인강화BMSC-Bio-Oss복합골수궤식입견일측상합두저제승형성적공강중위실험조,령일측식입BMSC-Bio-Oss복합골위공백대조조.분별우술후30、60、120 d행대체관찰、CT검사、조직학검사화신생골량분석.결과 라서피하가견신생골,형광현미경시종가견BMSC.견상합두저제승30 d후,대체관찰화CT검사시소량신골형성,120 d후상합두내신골생성치밀,부유소량Bio-Oss골.신생골량분석현시,여공백대조조[(19.67 ±5.73) mm2]상비,실험조신생골량[(24.74±6.33) mm2]현저증다(P＜0.05).결론 BMP-2기인강화BMSC-Bio-Oss복합골응용우상합두저제승술가획득량호적성골효과.
Objective To evaluate the effect of maxillary sinus elevation with gene-enhanced tissue engineering bone in dogs.Methods bone marrow stromal cells (BMSC) derived from dog marrow were cultured,and transduced with the adenovirus carrying bone morphogenetic protein-2(BMP-2) gene( AdBMP-2),the adenovirus carrying green fluorescent protein (GFP) gene (AdGFP) in vitro.The bone formation ability of gene modified BMSC with scaffold was examined in nude mice and in elevated maxillary sinus of dog. Student's t-test was used for statistical analysis with SPSS 11.0 software package. Results Gene transfection efficiency reached up to (83.95 ± 2.43 ) ％ as demonstrated by GFP expression.Ectopic bone formation was detected in nude mice.As for maxillary sinus floor elevation in a dog model,new bone formation area in the AdBMP-2 gene transduced BMSC with Bio-Oss group was significantly higher than in BMSC with Bio-Oss group at 120 d (P ＜ 0.05 ).Conclusions AdBMP-2 gene transduced BMSC can stimulate ectopic bone formation in nude mice,and promote bone formation and maturation in the dog maxillary sinus.
