中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2011年
4期
239-243
,共5页
刘涛%李琳%贾麾%荆宏伟%孔垂泽
劉濤%李琳%賈麾%荊宏偉%孔垂澤
류도%리림%가휘%형굉위%공수택
RNA干扰%iASPP%肿瘤抑制蛋白质p53%膀胱肿瘤%癌
RNA榦擾%iASPP%腫瘤抑製蛋白質p53%膀胱腫瘤%癌
RNA간우%iASPP%종류억제단백질p53%방광종류%암
RNA interference%iASPP%Tumor suppressor protein p53%Urinary bladder neoplasms%Carcinoma
目的 探讨RNA干扰使p53抑制因子iASPP表达下调对膀胱癌细胞的影响.方法 iASPP siRNA慢病毒感染入膀胱癌细胞株5637和T24,实时定量PCR和蛋白质印迹法检测iASPP的表达;噻唑盐法测定细胞生长;集落形成测定法检测集落形成比率;荧光激活细胞分选术检测细胞周期.结果 iASPP siRNA慢病毒感染后细胞iASPP mRNA为0.60±0.02,低于阴性对照慢病毒组(1.00±0.03,P＜0.05).膀胱癌细胞iASPP蛋白表达降低.iASPP下调能抑制入膀胱癌细胞5637和T24的生长和增殖(P＜0.05),每个集落中细胞数显著减少(P＜0.05),集落数目也低于阴性对照组(P＜0.05).iASPP siRNA慢病毒5637细胞培养中的G1期细胞比率明显高于阴性对照慢病毒组,分别为(51.94±0.98)%和(46.00±0.77)%;T24细胞结果与之类似,分别为(60.04±0.45)%和(53.62±0.69)%;组间比较差异均有统计学意义(P＜0.05).结论 RNA干扰能够使膀胱癌细胞iASPP表达下调,从而抑制膀胱癌细胞的生长和增殖.
目的 探討RNA榦擾使p53抑製因子iASPP錶達下調對膀胱癌細胞的影響.方法 iASPP siRNA慢病毒感染入膀胱癌細胞株5637和T24,實時定量PCR和蛋白質印跡法檢測iASPP的錶達;噻唑鹽法測定細胞生長;集落形成測定法檢測集落形成比率;熒光激活細胞分選術檢測細胞週期.結果 iASPP siRNA慢病毒感染後細胞iASPP mRNA為0.60±0.02,低于陰性對照慢病毒組(1.00±0.03,P＜0.05).膀胱癌細胞iASPP蛋白錶達降低.iASPP下調能抑製入膀胱癌細胞5637和T24的生長和增殖(P＜0.05),每箇集落中細胞數顯著減少(P＜0.05),集落數目也低于陰性對照組(P＜0.05).iASPP siRNA慢病毒5637細胞培養中的G1期細胞比率明顯高于陰性對照慢病毒組,分彆為(51.94±0.98)%和(46.00±0.77)%;T24細胞結果與之類似,分彆為(60.04±0.45)%和(53.62±0.69)%;組間比較差異均有統計學意義(P＜0.05).結論 RNA榦擾能夠使膀胱癌細胞iASPP錶達下調,從而抑製膀胱癌細胞的生長和增殖.
목적 탐토RNA간우사p53억제인자iASPP표체하조대방광암세포적영향.방법 iASPP siRNA만병독감염입방광암세포주5637화T24,실시정량PCR화단백질인적법검측iASPP적표체;새서염법측정세포생장;집락형성측정법검측집락형성비솔;형광격활세포분선술검측세포주기.결과 iASPP siRNA만병독감염후세포iASPP mRNA위0.60±0.02,저우음성대조만병독조(1.00±0.03,P＜0.05).방광암세포iASPP단백표체강저.iASPP하조능억제입방광암세포5637화T24적생장화증식(P＜0.05),매개집락중세포수현저감소(P＜0.05),집락수목야저우음성대조조(P＜0.05).iASPP siRNA만병독5637세포배양중적G1기세포비솔명현고우음성대조만병독조,분별위(51.94±0.98)%화(46.00±0.77)%;T24세포결과여지유사,분별위(60.04±0.45)%화(53.62±0.69)%;조간비교차이균유통계학의의(P＜0.05).결론 RNA간우능구사방광암세포iASPP표체하조,종이억제방광암세포적생장화증식.
Objective To discuss the effects of silencing of iASPP gene on human bladder cancer cells. Methods RNAi silencing of iASPP gene in bladder cancer cell 5637 and T24 cells were used by lentiviral mediated interfering short hairpin RNAs. Cell proliferation was tested by MTT assay, and rate of colony was tested by colony formation assay. Cell cycles were tested by using fluorescence-activated cell sorting. Results Down-regulation of iASPP could inhibit the growth and proliferation of human bladder cancer cells (P＜0.05). iASPP know-down could decrease the colony formation of 5637 and T24 cells (P＜0, 05). Knocking down of iASPP in 5637 and T24 cells showed cell arrested at G1. Conclusions Silencing of iASPP gene could inhibit proliferation and colony formation of bladder cancer, iASPP might be an important target for gene therapy of bladder cancer.
