中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2009年
12期
817-819
,共3页
肾综合征性出血热%T淋巴细胞%免疫,细胞
腎綜閤徵性齣血熱%T淋巴細胞%免疫,細胞
신종합정성출혈열%T림파세포%면역,세포
Heamorrhagic fever with renal syndrome%T-lymphocytes%Immunity,cellular
目的 研究CD4+CD25+Foxp3+调节性T细胞在肾综合征出血热(HRFS)患者外周血中的比例改变并探讨其在疾病进程中的意义.方法 选取27例HRFS患者,外周血用细胞内染色的流式细胞术及荧光定量PCR的方法,分别在蛋白质和mRNA水平检测Foxp3+表达,并与肾活检结果及肾功能进行相关分析.同时取19名正常健康体检者作为正常对照组.结果 HRFS患者各期CD4+CD25+Foxp3+T细胞占CD4+T细胞比例明显均低于正常对照组(低血压休克期:5.0%±1.2%,少尿期:3.1%±0.8%,多尿期:4.2%±0.9%,恢复期:5.0%±1.1%,正常对照组:6.5%±2.1%,均P<0.01);各期间CD4+CD25+Foxp3+T细胞占CD4+T细胞比例差异亦有统计学意义,以少尿期为最少(P<0.01).CD4+CD25+Foxp3+T细胞占CD4+T细胞比例与肾组织损害指数显著相关(r=0.51,P<0.01),但与蛋白尿程度及肾功能损害无显著相关(r=0.11,0.18;均P>0.05).HRFS患者少尿期外周血单个核细胞中Foxp3 mRNA表达水平(503±64)明显低于正常对照组(3102±409)和其他期(低血压休克期:1805±291;多尿期:1869±320;恢复期:2697±420,均P<0.01).结论 HRFS患者CD4+CD25+Foxp3+T细胞明显减少,这群调节性T细胞可能参与了HRFS的病理进程.
目的 研究CD4+CD25+Foxp3+調節性T細胞在腎綜閤徵齣血熱(HRFS)患者外週血中的比例改變併探討其在疾病進程中的意義.方法 選取27例HRFS患者,外週血用細胞內染色的流式細胞術及熒光定量PCR的方法,分彆在蛋白質和mRNA水平檢測Foxp3+錶達,併與腎活檢結果及腎功能進行相關分析.同時取19名正常健康體檢者作為正常對照組.結果 HRFS患者各期CD4+CD25+Foxp3+T細胞佔CD4+T細胞比例明顯均低于正常對照組(低血壓休剋期:5.0%±1.2%,少尿期:3.1%±0.8%,多尿期:4.2%±0.9%,恢複期:5.0%±1.1%,正常對照組:6.5%±2.1%,均P<0.01);各期間CD4+CD25+Foxp3+T細胞佔CD4+T細胞比例差異亦有統計學意義,以少尿期為最少(P<0.01).CD4+CD25+Foxp3+T細胞佔CD4+T細胞比例與腎組織損害指數顯著相關(r=0.51,P<0.01),但與蛋白尿程度及腎功能損害無顯著相關(r=0.11,0.18;均P>0.05).HRFS患者少尿期外週血單箇覈細胞中Foxp3 mRNA錶達水平(503±64)明顯低于正常對照組(3102±409)和其他期(低血壓休剋期:1805±291;多尿期:1869±320;恢複期:2697±420,均P<0.01).結論 HRFS患者CD4+CD25+Foxp3+T細胞明顯減少,這群調節性T細胞可能參與瞭HRFS的病理進程.
목적 연구CD4+CD25+Foxp3+조절성T세포재신종합정출혈열(HRFS)환자외주혈중적비례개변병탐토기재질병진정중적의의.방법 선취27례HRFS환자,외주혈용세포내염색적류식세포술급형광정량PCR적방법,분별재단백질화mRNA수평검측Foxp3+표체,병여신활검결과급신공능진행상관분석.동시취19명정상건강체검자작위정상대조조.결과 HRFS환자각기CD4+CD25+Foxp3+T세포점CD4+T세포비례명현균저우정상대조조(저혈압휴극기:5.0%±1.2%,소뇨기:3.1%±0.8%,다뇨기:4.2%±0.9%,회복기:5.0%±1.1%,정상대조조:6.5%±2.1%,균P<0.01);각기간CD4+CD25+Foxp3+T세포점CD4+T세포비례차이역유통계학의의,이소뇨기위최소(P<0.01).CD4+CD25+Foxp3+T세포점CD4+T세포비례여신조직손해지수현저상관(r=0.51,P<0.01),단여단백뇨정도급신공능손해무현저상관(r=0.11,0.18;균P>0.05).HRFS환자소뇨기외주혈단개핵세포중Foxp3 mRNA표체수평(503±64)명현저우정상대조조(3102±409)화기타기(저혈압휴극기:1805±291;다뇨기:1869±320;회복기:2697±420,균P<0.01).결론 HRFS환자CD4+CD25+Foxp3+T세포명현감소,저군조절성T세포가능삼여료HRFS적병리진정.
Objective To investigate the percentage and significance of CD4+ CD25+ Foxp3+ regulatory T cells in the patients with heamorrhagic fever with renal syndrome (HFRS). Methods Peripheral blood samples were collected from 27 HFRS patients, and 19 healthy controls to examine the proportion of the CD4+ CD25+Foxp3 + cells. RT-PCR was used to detect the Foxp3 mRNA expression in the mononuclear cells in peripheral blood. Biopsy was conducted on the HFRS patients during oliguria stage. Peripheral blood samples and morning urine samples were collected in the stages and in the very day of renal biopsy to examine the urine protein, urine creatinine (Cr), blood Cr, and creatinine clearance rate (Ccr). Results The percentages of CD4+ CD25+ Foxp3+> in the HFRS patients in the hypetension, oliguria, polyuria, and convalescent stages were 5.0% ± 1.2%, 3. 1%± 0. 8%, 4. 2% ± 0. 9%, and 5.0% 4± 1.1% respectively, all significantly lower than that of the normal control group (6. 5% ± 2. 1%, all P < 0. 01). The proportion of CD4+ CD25+ Foxp3 +> T cells in the CD4 +> T cells in the oliguria stage was significantly lower than those in other stages and that of the normal controls (P <0. 01). The percentage of CD4 + CD25 + Foxp3 + in the patients with HFRS was significantly correlated with the renal injury index (r = 0. 51, P <0. 01), but not significantly correlated with urine protein and renal function changes (r =0. 11 and 0. 18, both P > 0. 05). The expression level of Foxp3 mRNA in peripheral mononuclear cells of the HEFS patients in the oliguria stage was (503 ± 64), significantly lower than that of the normal controls (3102 ±409, P <0. 01) and those of the HFRS patients in the hypotension, polyuria, and convalescent stages (1805 ±291, 1869 ±320, and 2697±420,all P <0.01). Conclusion The proportion of CD4+ CD25+ Foxp3 + regulatory T cells decreases in patients with HFRS, which may contribute to the pathological process in such disease.
