CAJ | 학술논문

目的建立灵敏、专属的HPLC-ESI-MS测定法, 用于测定家兔口服灯盏花素缓释片(2片 60 mg/片)后的血药浓度.方法该定量方法采用芦丁作内标,用固相萃取(SPE)进行样品前处理.以甲醇10 mmol·L-1醋酸铵水溶液(pH 8.0)梯度洗脱,流动相流速0.4 mL·min-1,柱温35 ℃,经过Zorbax Extend-C18 (150 mm×2.1 mm ID, 5 μm)色谱柱分离,电喷雾质谱检测器在负离子模式下检测灯盏乙素和芦丁.结果在2～200 ng·mL-1范围内灯盏乙素与内标峰面积与浓度线性关系良好,且准确度、精密度满足生物样品分析的要求.结论本实验建立的兔血浆中灯盏乙素的HPLC-MS分析方法灵敏度高,可用于口服给药后灯盏乙素的血药浓度测定.
목적건립령민、전속적HPLC-ESI-MS측정법, 용우측정가토구복등잔화소완석편(2편 60 mg/편)후적혈약농도.방법해정량방법채용호정작내표,용고상췌취(SPE)진행양품전처리.이갑순10 mmol·L-1작산안수용액(pH 8.0)제도세탈,류동상류속0.4 mL·min-1,주온35 ℃,경과Zorbax Extend-C18 (150 mm×2.1 mm ID, 5 μm)색보주분리,전분무질보검측기재부리자모식하검측등잔을소화호정.결과재2～200 ng·mL-1범위내등잔을소여내표봉면적여농도선성관계량호,차준학도、정밀도만족생물양품분석적요구.결론본실험건립적토혈장중등잔을소적HPLC-MS분석방법령민도고,가용우구복급약후등잔을소적혈약농도측정.
Aim To establish a sensitive and specific liquid chromatography-mass spectrometry method for determination of scutellarin in rabbit plasma after oral administration. Methods For the quantitative analysis, rutin was used as an internal standard and solid-phase extraction (SPE) was performed by using a Phenomenex C8 cartridge. HPLC was carried out using a Zorbax Extend-C18column (150 mm×2.1 mm ID, 5 μm) with a guard cartridge (Phenomenex). Gradient elution was selected with the mobile phase of methanol 10 mmol·L-1 ammonium acetate solution (pH adjusted to 8.0 with ammonia solution). The flow rate of mobile phase was 0.4 mL·min-1 and the column temperature was 35 ℃. Both scutellarin and the internal standard rutin in rabbit plasma extracts were detected by mass spectrometry using an ESI interface in the negative ion mode. Results The linear range was from 2 to 200 ng·mL-1, with acceptable accuracy and precision (RSD). Conclusion A sensitive, reliable and accurate method for the quantitation of scutellarin in rabbit plasma has been established.