CAJ | 학술논문

目的:种间胚胎移植是挽救濒危动物的一个有效手段.以往的研究主要集中于对种间、同种妊娠之间微观结构和解剖学差异的描述上,而对导致这些现象产生的分子机制的研究却所见甚少.本研究的目的是阐明同种妊娠和科间妊娠之间免疫反应的差异,并初步探讨科间妊娠中流产的可能原因.方法:以科间妊娠第4天、第8天、第12天的孕体为实验组,小鼠同种移植妊娠第4天、第8天、第12天的子宫孕体和相应时间假孕小鼠的子宫为阳性和阴性对照.结果:采用间接免疫荧光和激光共聚焦扫描的方法对冰冻切片显色的结果表明:妊娠第8天时,MHCⅡ分子和Gal-α-Gal抗原决定簇的表达位置和表达密度在科间妊娠、阳性、阴性对照之间均不相同.妊娠第八天时,MHCⅡ分子在科间妊娠中的表达弱于同种妊娠,其表达模式在第12天时也不相同.Gal-α-Gal抗原决定簇在科间妊娠第8天时不表达,但在同种妊娠中却有一定水平的表达.在妊娠第12天,科间妊娠中有一定量的表达,但与同种妊娠的表达模式不同.结论:科间妊娠和同种妊娠的免疫反应有差异,这些差异可能是造成科间妊娠失败的原因之一.
목적:충간배태이식시만구빈위동물적일개유효수단.이왕적연구주요집중우대충간、동충임신지간미관결구화해부학차이적묘술상,이대도치저사현상산생적분자궤제적연구각소견심소.본연구적목적시천명동충임신화과간임신지간면역반응적차이,병초보탐토과간임신중유산적가능원인.방법:이과간임신제4천、제8천、제12천적잉체위실험조,소서동충이식임신제4천、제8천、제12천적자궁잉체화상응시간가잉소서적자궁위양성화음성대조.결과:채용간접면역형광화격광공취초소묘적방법대빙동절편현색적결과표명:임신제8천시,MHCⅡ분자화Gal-α-Gal항원결정족적표체위치화표체밀도재과간임신、양성、음성대조지간균불상동.임신제팔천시,MHCⅡ분자재과간임신중적표체약우동충임신,기표체모식재제12천시야불상동.Gal-α-Gal항원결정족재과간임신제8천시불표체,단재동충임신중각유일정수평적표체.재임신제12천,과간임신중유일정량적표체,단여동충임신적표체모식불동.결론:과간임신화동충임신적면역반응유차이,저사차이가능시조성과간임신실패적원인지일.
Subjective: Interspecific embryo transfer could be a valuable tool in preservation programs of endangered species. The majority of previous studies focused on descriptions of microstructure and anatomic differences between intraspecific and interspecific pregnancy, while the report for molecular mechanism of these phenomena was few. The aim of this work was to illuminate the difference of immunological reaction between intraspecific and interfamily pregnancy and to primarily grope for the possible reasons of interfamily pregnant abortion. Methods: The fetuses of interfamily pregnancy day 4, day 8 and day 12 were acted as experimental group, while the ones of intraspecific pregnancy day 4, day 8 and day 12 of embryo transfer and the uteri of pseudopregnant mice of corresponding time were employed as positive and negative groups respectively. Results: The indirect immunofluorescence and laser confocal scan results of cryosections showed: Expressive positions and intensities of MHC class Ⅱ molecules and Gal-α-Gal epitopes were all different in interfamily pregnancy, positive and negative controls in pregnancy day 8. The expressions of MHC class Ⅱ molecules in interfamily pregnancy were weaker than in intraspecfic pregnancy in day 8, and expressive modes in day 12 were also different. Gal-α-Gal epitopes did not expresse in interfamily pregnancy day 8, but in intraspecific pregnancy they expressed in a certain level. In pregnancy day 12, they expressed in a certain degree in interfamily pregnancy, but their expressive modes were different to those in intraspecific pregnancy. Conclusion: These results indicated the differences between interfamily and intraspecific pregnancy. It was possible that these different expressions of MHC class Ⅱ molecules and Gal-α-Gal epitopes would be one of the reasons leading to the failure in interfamily pregnancy.