CAJ | 학술논문

为建立2型猪链球菌(S.suis 2)胞外蛋白组双向电泳样品的制备方法,本研究利用双向电泳(2-DE):分离S.suis 2强毒株与无致病株的胞外蛋白,分别得到它们的胞外蛋白图谱.在pH4～pH7范围内采用考马斯亮蓝R350染色法在2菌株的胞外蛋白质图谱中都分别检测出180±10个蛋白点.并且它们的蛋白质分子质量分布基本相似;在所检测到差异蛋白点中,其中有50个蛋白点只存在于无致病菌株中而强毒株中不存在,有52个蛋白点只存在于强毒株中而无致病菌株中不存在,有7个蛋白点在2菌株中的表达量相差5倍以上.我们在强毒株凝胶中挑取了10个差异蛋白点进行质谱分析,通过数据库检索,鉴定了其中的9个蛋白,另外1种与鞭毛蛋白有同源序列.这些结果为研究S.suis2的致病机理提供了蛋白质组学方面的信息.
위건립2형저련구균(S.suis 2)포외단백조쌍향전영양품적제비방법,본연구이용쌍향전영(2-DE):분리S.suis 2강독주여무치병주적포외단백,분별득도타문적포외단백도보.재pH4～pH7범위내채용고마사량람R350염색법재2균주적포외단백질도보중도분별검측출180±10개단백점.병차타문적단백질분자질량분포기본상사;재소검측도차이단백점중,기중유50개단백점지존재우무치병균주중이강독주중불존재,유52개단백점지존재우강독주중이무치병균주중불존재,유7개단백점재2균주중적표체량상차5배이상.아문재강독주응효중도취료10개차이단백점진행질보분석,통과수거고검색,감정료기중적9개단백,령외1충여편모단백유동원서렬.저사결과위연구S.suis2적치병궤리제공료단백질조학방면적신식.
The extracellular proteins of Streptococcus suis type 2 virulent strain and avirulent strain was separated by two-dimensional electrophoresis (2-DE) and the 2-DE profiles were compared. The results showed that both strains have 180±10 protein spots with similar molecular masses. Howevers there were about 50 protein spots in avirulent strain not displayed in the virulent strain, and 52 protein spots in virulent strain not displayed in avirulent strain. There were 7 spots showed more than 5 folds expression changes between the two strains. Ten mis-matched spots in the 2-DE map of S. Suis type 2 virulent strain were cut out and 9 proteins were successfully identified by MALDI-TOF-MS and/or MALDI-TOF/TOF-MS. One protein showed homologous sequence with flagellin. These proteomic results would provide insight into further understanding of pathogenic mechanism of S. Suis type 2.