CAJ | 학술논문

目的:观察大鼠脊髓损伤后及进行微囊化兔雪旺细胞移植后胶质纤维酸性蛋白(GFAP)表达的变化.方法: 130只成年SD大鼠随机分为微囊组、细胞悬液组、损伤对照组和正常对照组4组,术后3、 7、 14及28d,冰冻切片行免疫组织化学显色观察GFAP表达的变化.结果:大鼠脊髓损伤后3～14d, GFAP阳性细胞数及平均光密度均增加;至第28天时则减少,但仍高于正常组.其中阳性细胞数和平均光密度在第7天开始,微囊组与细胞悬液组、损伤组比较均有明显降低.结论: 微囊化异种雪旺细胞移植能抑制损伤脊髓GFAP的表达,减轻由反应性胶质化所形成的胶质瘢痕.
목적:관찰대서척수손상후급진행미낭화토설왕세포이식후효질섬유산성단백(GFAP)표체적변화.방법: 130지성년SD대서수궤분위미낭조、세포현액조、손상대조조화정상대조조4조,술후3、 7、 14급28d,빙동절편행면역조직화학현색관찰GFAP표체적변화.결과:대서척수손상후3～14d, GFAP양성세포수급평균광밀도균증가;지제28천시칙감소,단잉고우정상조.기중양성세포수화평균광밀도재제7천개시,미낭조여세포현액조、손상조비교균유명현강저.결론: 미낭화이충설왕세포이식능억제손상척수GFAP적표체,감경유반응성효질화소형성적효질반흔.
Objective: To study the changes of glial fibrillary acidic protein (GFAP) expression after spinal cord injury (SCI) and the changes after transplantation of microencapsulated Schwann cells. Methods: 120 adult Sprague-Dawley rats were randomly and equally divided into microcapsule group, cell suspension group, injury group and control group. Additional 10 rats were used as normal controls. The expression of GFAP in sections of rat spinal cord was detected using immnohistochemical technique at 3, 7, 14 and 28 days after injury. Results: After SCI, the number of GFAP positive cells and mean optical density of GFAP were raised from day 3 to day 14, and decreased on day 28, which was still higher than normal. Compared with cell suspension and injury groups, xenotransplantation of microencapsulated Schwann cells produced a significant decrease in the number of GFAP positive cells and mean optical density of GFAP. Conclusion: Xenotransplantation of microencapsulated Schwann cells can downregulate the expression of GFAP in astrocytes of the injured spinal cord, therefore, reduce glial scar caused by reactive astrogliosis.