CAJ | 학술논문

采用超临界CO_2 流体萃取技术脱除黄花忍冬果中脂类及色素等物质后,用蒸馏水浸提法提取其中多糖并采用苯酚-硫酸法测定多糖的含量.超临界 CO_2 流体萃取的条件为:萃取压力 30 MPa、萃取温度35 ℃、萃取时间 4 h、CO_2 流量 25 L/h.萃取后所得黄花忍冬果粉末为淡黄色,风味纯正,以其为原料提取黄花忍冬果多糖,多糖色泽乳白,无异味.测得黄花忍冬果中多糖的含量是14.32 %,平均回收率为99.28 %,RSD=1.68 %.通过实验结果表明,本实验方法可行,可作为黄花忍冬果中多糖的提取与检测的方法.
채용초림계CO_2 류체췌취기술탈제황화인동과중지류급색소등물질후,용증류수침제법제취기중다당병채용분분-류산법측정다당적함량.초림계 CO_2 류체췌취적조건위:췌취압력 30 MPa、췌취온도35 ℃、췌취시간 4 h、CO_2 류량 25 L/h.췌취후소득황화인동과분말위담황색,풍미순정,이기위원료제취황화인동과다당,다당색택유백,무이미.측득황화인동과중다당적함량시14.32 %,평균회수솔위99.28 %,RSD=1.68 %.통과실험결과표명,본실험방법가행,가작위황화인동과중다당적제취여검측적방법.
After removing the lipid and pigment in Lonicera chrysantha fruit through supercritical CO_2 fluid extraction,the polysaccharide in Lonicera chrysantha fruit was extracted with distilled water and determined by Phenol-sulfuric acid method. The content of polysaccharide in Lonicera chrysantha fruit was 14.32 %.The average recovery was 99.28%,RSD was 1.68%.The optimum conditions of supercritical CO_2 fluid extraction were that the pressure was 30Mpa,temperature was 35 ℃,time was 4 h, CO_2 flow was 25 L/h. The product was sulphureum and had the good flavor . The Lonicera chrysantha fruit polysaccharide extracted from the product was milk and had no strange smell. The experimental results showed that the experimental method was feasible and could be used for extracing and determinating the polysaccharide in Lonicera chrysantha fruit.