CAJ | 학술논문

采用DPPH·法及Fe~(3+)还原力法对蛇菰提取物的抗氧化活性进行研究,分别测定蛇菰乙醇提物、石油醚提取物、氯仿提取物、乙酸乙酯提取物、正丁醇提取物、水层部分、水提粗多糖及碱提粗多糖的抗氧化作用,同时以VC作为阳性对照.结果表明:蛇菰提取物具有较强的抗氧化活性,尤其是乙酸乙酯提取物、正丁醇提取物、总醇提取物及水提粗多糖;它们对DPPH·的清除作用与VC相当,其中乙酸乙酯提取物IC_50值为6.0μg/mL,活性略高于VC,同时以上提取物对Fe~(3+)也具有很强的还原能力.
채용DPPH·법급Fe~(3+)환원역법대사고제취물적항양화활성진행연구,분별측정사고을순제물、석유미제취물、록방제취물、을산을지제취물、정정순제취물、수층부분、수제조다당급감제조다당적항양화작용,동시이VC작위양성대조.결과표명:사고제취물구유교강적항양화활성,우기시을산을지제취물、정정순제취물、총순제취물급수제조다당;타문대DPPH·적청제작용여VC상당,기중을산을지제취물IC_50치위6.0μg/mL,활성략고우VC,동시이상제취물대Fe~(3+)야구유흔강적환원능력.
DPPH·free radical scavenging assay and ferric reducing power assay in vitro were used to evaluate the antioxidant activities of ethanol extract, petroleum ether extract, chloroform extract, ethyl acetate extract, n-butanol extract, water extraction fraction, water-soluble crude polysaccharides and alkaline-soluble crude polysaccharides from Balanophora spicata Hayata.Results indicated that the extracts from Balanophora spicata Hayata all exhibited strong scavenging capacity against DPPH free radicals, especially ethyl acetate extract, n-butanol extract ethanol extract and water-soluble crude polysaccharides, which were nearly equivalent to vitamin C for DPPH free radical scavenging activity. The fact that ethyl acetate extract exhibited an IC_(50) value of 6.0 μg/mL suggested that a little stronger antioxidant activity of ethyl acetate extract than vitamin C. Meanwhile, the above extracts all revealed stronger ferric reducing power.