蛛形学报
蛛形學報
주형학보
ACTA ARACHNOLOGICA SINICA
2012年
1期
17-21
,共5页
寄生蚌螨%弯弓蚌螨%12S rRNA基因%隐藏种
寄生蚌螨%彎弓蚌螨%12S rRNA基因%隱藏種
기생방만%만궁방만%12S rRNA기인%은장충
Unionicolid mites%Unionicola arcuata%12S rRNA gene%cryptic species
Unionicola sp.与弯弓蚌螨U.arcuata在形态结构上非常相似,为了鉴别弯弓蚌螨U.arcuata 和Unionicola sp.,本研究扩增出Unionicola sp.12S rRNA的部分基因片段,与基因数据库中5种寄生蚌螨序列进行比较分析.结果显示,Unionicola sp.与弯弓蚌螨U.arcuata遗传差异为0.145,而分子系统树表明,Unionicola sp.与弯弓蚌螨U.arcuata聚为一支,具较近的亲缘关系.我们推断Unionicola sp.是弯弓蚌螨U.arcuata的1个隐藏种.这可能是由于不同寄主蚌造成了宿主隔离,从而引起了蚌螨遗传基因的分化.
Unionicola sp.與彎弓蚌螨U.arcuata在形態結構上非常相似,為瞭鑒彆彎弓蚌螨U.arcuata 和Unionicola sp.,本研究擴增齣Unionicola sp.12S rRNA的部分基因片段,與基因數據庫中5種寄生蚌螨序列進行比較分析.結果顯示,Unionicola sp.與彎弓蚌螨U.arcuata遺傳差異為0.145,而分子繫統樹錶明,Unionicola sp.與彎弓蚌螨U.arcuata聚為一支,具較近的親緣關繫.我們推斷Unionicola sp.是彎弓蚌螨U.arcuata的1箇隱藏種.這可能是由于不同寄主蚌造成瞭宿主隔離,從而引起瞭蚌螨遺傳基因的分化.
Unionicola sp.여만궁방만U.arcuata재형태결구상비상상사,위료감별만궁방만U.arcuata 화Unionicola sp.,본연구확증출Unionicola sp.12S rRNA적부분기인편단,여기인수거고중5충기생방만서렬진행비교분석.결과현시,Unionicola sp.여만궁방만U.arcuata유전차이위0.145,이분자계통수표명,Unionicola sp.여만궁방만U.arcuata취위일지,구교근적친연관계.아문추단Unionicola sp.시만궁방만U.arcuata적1개은장충.저가능시유우불동기주방조성료숙주격리,종이인기료방만유전기인적분화.
The morphological characters of Unionicola sp.and U.arcuata were very similar.To identify Unionicola sp.from U.arcuata,a portion of the mitochondrial 12S rRNA gene was amplified,cloned,and sequenced from Unionicola sp.,comparative sequence analysis with other five unionicolid mites of GenBank.The results indicated that coefficients of genetic distance of Unionicola sp.and U.arcuata were 0.145,and molecular tree showed that Unionicola sp.and U.arcuata were the sister groups.These results suggest that Unionicola sp.is a cryptic species of U.arcuata.It might be resulted in the host isolation for the different host clam,and genetic differentiation of unionicolid mites were influenced by the host species.