CAJ | 학술논문

目的研究EphB2在先天性肛门直肠畸形直肠末端组织中的表达并探讨其临床价值.方法采用免疫组织化学法和免疫荧光分析方法检测20例先天性肛门直肠畸形患儿直肠末端肠壁和8例正常对照直肠末端肠肇的EphB2的表达情况.结果免疫组织化学检测到EphB2蛋白在畸形组和对照组均有表达,定位在直肠肠壁黏膜层细胞的胞质和胞膜中.畸形组EphB2表达的累积光密度值(30.43±11.41)明显低于对照组(81.62±20.45),差异有统计学意义(P<0.01).结论 EphB2在肛门直肠畸形的直肠末端黏膜层表达水平减低,EphB2的表达可能和先天性肛门直肠畸形的发生密切相关.
목적 연구EphB2재선천성항문직장기형직장말단조직중적표체병탐토기림상개치.방법 채용면역조직화학법화면역형광분석방법검측20례선천성항문직장기형환인직장말단장벽화8례정상대조직장말단장조적EphB2적표체정황.결과 면역조직화학검측도EphB2단백재기형조화대조조균유표체,정위재직장장벽점막층세포적포질화포막중.기형조EphB2표체적루적광밀도치(30.43±11.41)명현저우대조조(81.62±20.45),차이유통계학의의(P<0.01).결론 EphB2재항문직장기형적직장말단점막층표체수평감저,EphB2적표체가능화선천성항문직장기형적발생밀절상관.
Objective The aim of this study is to evaluate the expression of EphB2 protein in terminal rectum with congenital anorectal malformation(CAM) and to assess its clinical significance. Methods Specimens were obtained from 20 patients with CAM, and 8 normal controls. Expression of EphB2 was determined by SP immunohistochemistry and immunofluorescence. Results The expression of EphB2 was mainly located in the cytoplasm and cell membrane of the mucous layer by immunohistochemistry. The positive expression intensity of EphB2 proteins were significantly lower in the malformation group (30.43 ± 11.41) than in the control group (81.62 ± 20.45) by integrated optical density(IOD) (P<0.01). Conclusions There was decreased expression of EphB2 in the mucous layer of CAM. Results suggested that EphB2