CAJ | 학술논문

目的探讨乙酰肝素酶(heparanase,Hpa)在非黑素性皮肤癌[基底细胞癌(BCC)和皮肤鳞状细胞癌(CSCC)]组织中的表达及其与CSCC病理分级和淋巴结转移的关系.方法采用RT-PCR法和免疫组化法检测38例CSCC、33例BCC及19例正常皮肤组织中Hpa的表达水平,分析Hpa的表达与CSCC临床病理特征的关系.结果 CSCC组织中Hpa mRNA、Hpa蛋白的阳性表达率(68.4%、60.5%)均显著高于它们在BCC组织(24.2%、18.2%)和正常皮肤组织(10.5%、5.3%)中的表达(均P<0.01);CSCC组织中有淋巴结转移(92.3%、84.6%)和Broders分类Ⅲ～Ⅳ级Hpa mRNA、Hpa蛋白的阳性表达率(92.3%、84.6%)明显高于无淋巴结转移(56.0%、48.0%)和Ⅰ、Ⅱ级(56.0%、48.0%)中的表达(均P<0.05).结论 CSCC组织中Hpa表达高于BCC组织和正常皮肤组织,并与CSCC病理分级和淋巴结转移有关.
목적 탐토을선간소매(heparanase,Hpa)재비흑소성피부암[기저세포암(BCC)화피부린상세포암(CSCC)]조직중적표체급기여CSCC병리분급화림파결전이적관계.방법 채용RT-PCR법화면역조화법검측38례CSCC、33례BCC급19례정상피부조직중Hpa적표체수평,분석Hpa적표체여CSCC림상병리특정적관계.결과 CSCC조직중Hpa mRNA、Hpa단백적양성표체솔(68.4%、60.5%)균현저고우타문재BCC조직(24.2%、18.2%)화정상피부조직(10.5%、5.3%)중적표체(균P<0.01);CSCC조직중유림파결전이(92.3%、84.6%)화Broders분류Ⅲ～Ⅳ급Hpa mRNA、Hpa단백적양성표체솔(92.3%、84.6%)명현고우무림파결전이(56.0%、48.0%)화Ⅰ、Ⅱ급(56.0%、48.0%)중적표체(균P<0.05).결론 CSCC조직중Hpa표체고우BCC조직화정상피부조직,병여CSCC병리분급화림파결전이유관.
Objective To investigate the expression of heparanase in non-melanoma skin cancer including cutaneous squamous cell carcinoma (CSCC) and basal cell carcinoma (BCC) and the relationship among heparanase expression and pathologic grading and lymph nodes metastasis of CSCC. Methods The expressions of heparanase were detected by RT-PCR assay and immunohistochemical staining in 38 cases of CSCC, 33 BCC and 19 normal human skin tissues. Then the relationship between heparanase expression and CSCC clinical pathological features was explored. Results The positive rate ( 68.4%, 60. 5% ) of heparanase mRNA and heparanase in CSCC (24. 2%, 18. 2% ) was significantly higher than those of BCC and normal skin tissue ( 10. 5% ,5.3% ) ( P <0. 01 ) . Heparanase expression rate in CSCC with lymph nodes metastasis (92. 3% ,84.6% ) and Ⅲ～Ⅳ(Broders) (92. 3% ,84. 6% ) was higher than those in tumor without lymph nodes metastasis (56. 0% ,48.0% ) and Ⅰ～Ⅱ (Broders) (56. 0% ,48. 0% ) ( P <0. 05). Conclusions Heparanase was over-expressed in CSCC compared with BCC and normal skin tissues. Expression of heparanase was correlated to pathologic grade and lymph nodes metastasis of CSCC.