中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2010年
7期
751-753
,共3页
叶春梅%罗凤玲%薛明兴%陈波%黄自明%赵松%王蓉
葉春梅%囉鳳玲%薛明興%陳波%黃自明%趙鬆%王蓉
협춘매%라봉령%설명흥%진파%황자명%조송%왕용
乳腺肿瘤%前哨淋巴结%微转移%逆转录PCR%蛋白质印迹%细胞角蛋白19
乳腺腫瘤%前哨淋巴結%微轉移%逆轉錄PCR%蛋白質印跡%細胞角蛋白19
유선종류%전초림파결%미전이%역전록PCR%단백질인적%세포각단백19
Breast neoplasms%Sentinel lympk node%Micrometastasis%RT-PCR%Western Blot%CK19
目的 检测乳腺癌前哨淋巴结(SIN)组织中细胞角蛋白19(CK19)在mRNA和蛋白水平的表达情况,以探讨检测淋巴结CK19的表达对预测乳腺癌前哨淋巴结微转移的临床价值.方法 本组30例可手术的原发性乳腺癌患者,手术中均行肿块周围或乳晕周围注射亚甲蓝进行SLN定位成功,同时常规行腋窝淋巴结清扫,术后对SLN和非SLN均行常规病理学检测,对SLN同时采用RT-PCR和Western Blot方法检测CK19的表达.结果 30例SLN常规病理检测发现8例阳性SLN,阳性率为26.67%(8/30),1例假阴性.应用RT-PCR方法和Western Blot方法检测30例SLN中CK19的表达,结果HE染色阳性的8例SLN及1例假阴性SLN均呈阳性表达,共有12例SLN CK19mRNA表达阳性,阳性率为40.0%(12/30);Western Blot检测发现1I例SLN CK19呈阳性,阳性率为36.67%(11/30).RT-PCR、Western Blot检测结果分别与HE染色结果比较,差异均有统计学意义(P<0.05),假阴性率为0.结论 CK19mRNA可作为标志物来检测乳腺癌SLN中的微转移情况;RT-PCR方法可检测到SLN微转移灶,通过SLN定位后检测其中CK19mRNA的阳性表达,可明显提高乳腺癌SLN的阳性率,降低SLN单纯病理检测的假阴性率.
目的 檢測乳腺癌前哨淋巴結(SIN)組織中細胞角蛋白19(CK19)在mRNA和蛋白水平的錶達情況,以探討檢測淋巴結CK19的錶達對預測乳腺癌前哨淋巴結微轉移的臨床價值.方法 本組30例可手術的原髮性乳腺癌患者,手術中均行腫塊週圍或乳暈週圍註射亞甲藍進行SLN定位成功,同時常規行腋窩淋巴結清掃,術後對SLN和非SLN均行常規病理學檢測,對SLN同時採用RT-PCR和Western Blot方法檢測CK19的錶達.結果 30例SLN常規病理檢測髮現8例暘性SLN,暘性率為26.67%(8/30),1例假陰性.應用RT-PCR方法和Western Blot方法檢測30例SLN中CK19的錶達,結果HE染色暘性的8例SLN及1例假陰性SLN均呈暘性錶達,共有12例SLN CK19mRNA錶達暘性,暘性率為40.0%(12/30);Western Blot檢測髮現1I例SLN CK19呈暘性,暘性率為36.67%(11/30).RT-PCR、Western Blot檢測結果分彆與HE染色結果比較,差異均有統計學意義(P<0.05),假陰性率為0.結論 CK19mRNA可作為標誌物來檢測乳腺癌SLN中的微轉移情況;RT-PCR方法可檢測到SLN微轉移竈,通過SLN定位後檢測其中CK19mRNA的暘性錶達,可明顯提高乳腺癌SLN的暘性率,降低SLN單純病理檢測的假陰性率.
목적 검측유선암전초림파결(SIN)조직중세포각단백19(CK19)재mRNA화단백수평적표체정황,이탐토검측림파결CK19적표체대예측유선암전초림파결미전이적림상개치.방법 본조30례가수술적원발성유선암환자,수술중균행종괴주위혹유훈주위주사아갑람진행SLN정위성공,동시상규행액와림파결청소,술후대SLN화비SLN균행상규병이학검측,대SLN동시채용RT-PCR화Western Blot방법검측CK19적표체.결과 30례SLN상규병리검측발현8례양성SLN,양성솔위26.67%(8/30),1례가음성.응용RT-PCR방법화Western Blot방법검측30례SLN중CK19적표체,결과HE염색양성적8례SLN급1례가음성SLN균정양성표체,공유12례SLN CK19mRNA표체양성,양성솔위40.0%(12/30);Western Blot검측발현1I례SLN CK19정양성,양성솔위36.67%(11/30).RT-PCR、Western Blot검측결과분별여HE염색결과비교,차이균유통계학의의(P<0.05),가음성솔위0.결론 CK19mRNA가작위표지물래검측유선암SLN중적미전이정황;RT-PCR방법가검측도SLN미전이조,통과SLN정위후검측기중CK19mRNA적양성표체,가명현제고유선암SLN적양성솔,강저SLN단순병리검측적가음성솔.
Objective To detect the expression of CK19 in sentinel lymph node for breast cancer and to evaluate its clinical significance.Methods SLNs of 30 patients with breast cancer were successfully detected with methylene blue,both SLN and ALN were examined by hematoxylin and eosin staining ( HK staining). The expression level of CK19 in SLNs were assessed by RT-PCR and Western Blot for mRNA and Protein respectively. Results Detection positive rate of SLN melastases was 26. 67% (8/30) by HE staining,and 1 case of false negative. Through RT-PCR,12 cases,including all the positive cases detected by routine pathological examination,were detected to be positive in the expression of CK19mRNA, with a positive rate of 40. 0% ( 12/30) , and a positive rate of 36. 67% (11/30) for CK19 was observed by Western Blot There was a significant difference between RT-PCR or Western Blot methods and routine pathological examination ( P < 0.05 ) for the detection efficiency of SI.Ns melastases. Conclusions CK19rnRNA may be a potential tumor marker for detecting micrometastasis in SLNs of breast cancer. The combined utilization of locating SLNs and detecting CK19 mRNA expression by RT-PCR instead of histopathological examination can greatly enhance the detection efficiency of SLN micrometastasis of breast cancer.
