CAJ | 학술논문

目的探讨中国华东地区汉族人群HLA-DRB1基因与斑秃发病、临床特点的关系.方法采用序列特异性引物PCR(PCR-SSP)技术对已确诊为斑秃的158例和正常人对照组172例进行HLA-DRB1基因多态性分析.并比较斑秃患者不同发病年龄、发病次数、病程、家族史及严重程度与HLA-DRB1基因的关联性.结果斑秃组HLA-DRB1*03、HLA-DRB1*11等位基因频率与对照组差异无统计学意义.斑秃组HLA-DRB1*04(OR=1.99,Pc=0.01)等位基因频率明显高于对照组.与正常人对照组比较,斑秃晚发组(发病年龄＞16岁)(OR=1.94,Pc=0.02)、斑秃复发组(发病次数＞1)和初发组(OR=2.49、Pc=0.02,OR=1.83、Pc=0.04)、病程＞1年的患者(OR=2.94,Pc=0.01)、无家族史的患者(OR=1.97,Pc=0.02)、严重斑秃患者(OR=3.53,Pc=0.00)HLA-DRB1*04等位基因频率均显著升高.结论中国华东地区汉族人群HLA-DRB1*04等位基因与斑秃发病、临床分型显著相关.
목적 탐토중국화동지구한족인군HLA-DRB1기인여반독발병、림상특점적관계.방법 채용서렬특이성인물PCR(PCR-SSP)기술대이학진위반독적158례화정상인대조조172례진행HLA-DRB1기인다태성분석.병비교반독환자불동발병년령、발병차수、병정、가족사급엄중정도여HLA-DRB1기인적관련성.결과 반독조HLA-DRB1*03、HLA-DRB1*11등위기인빈솔여대조조차이무통계학의의.반독조HLA-DRB1*04(OR=1.99,Pc=0.01)등위기인빈솔명현고우대조조.여정상인대조조비교,반독만발조(발병년령＞16세)(OR=1.94,Pc=0.02)、반독복발조(발병차수＞1)화초발조(OR=2.49、Pc=0.02,OR=1.83、Pc=0.04)、병정＞1년적환자(OR=2.94,Pc=0.01)、무가족사적환자(OR=1.97,Pc=0.02)、엄중반독환자(OR=3.53,Pc=0.00)HLA-DRB1*04등위기인빈솔균현저승고.결론 중국화동지구한족인군HLA-DRB1*04등위기인여반독발병、림상분형현저상관.
Objective To investigate the association of HLA-DRB31*03,*04 and *11 alleles with alopecia areata(AA)in Han Nationality in East China.Methods Polymerase chain reaction-sequence specific primer(PCR-SSP)method was conducted in 158 Chinese Han patients with AA as well as in 172 healthy human controls in East China.The relationships of HLA-DRB1 polymorphism to age of onset,episode frequency,clinical course,family history,and severity of AA were evaluated.Results No significant differences were observed for the frequency of HLA DRB1*03,*11 alleles between the patients and human controls,while increased frequency of HLA-DRB1*04 was observed in patients(OR=1.99,Pc=0.01).Multiple logistic regression analysis revealed that HLA-DRB1*04 was more prevalent in patients with an onset after 16 years of age(OR=1.94,Pc=0.02),those without family history(OR=1.97,Pc=0.02),those with recurrent AA(OR=2.49,Pc=0.02),those with a clinical course of more than 1 year(OR=2.94,Pc=0.01),those with severe AA(OR=3.53,Pc=0.00)and tbose with single episode of AA(OR=1.83,Pc=0.04)in comparison with the normal human controls.Conclusion This study demonstrates that HLA-DRB1*04 allele is associated with the occurrence and clinical types of AA in Han Nationality in East China.