CAJ | 학술논문

目的探讨脊髓趋化因子配体21(CCL21)在大鼠胫骨癌痛中的作用.方法健康成年雌性SD大鼠48只,体重160～ 180 g,10～ 11周龄,采用随机数字表法,将大鼠随机分为6组(n=8):假手术组(Ⅰ组)、假手术+ CCL21中和抗体组(Ⅱ组)、骨癌痛组(Ⅲ组)、骨癌痛+溶媒PBS组(Ⅳ组)、骨癌痛+对照IgG组(Ⅴ组)和骨癌痛+CCI21中和抗体组(Ⅵ组).采用胫骨骨髓腔内注射Walker-256乳腺癌细胞方法制备胫骨癌痛模型.注射Walker-256乳腺癌细胞后14 d,Ⅳ组、Ⅴ组和Ⅵ组分别经L5,6鞘内注射PBS 15μl、亲和纯化的正常羊IgG 10 μg( 15 μl)和CCL21中和抗体10μg(15μl);Ⅱ组鞘内注射CCL21中和抗体10 μg.于注射Walker-256乳腺癌细胞或生理盐水前1 d(基础状态)、注射后7、14 d及鞘内给药后0.5、1、2、4、8、12、24、48 h(T1～10)时测定机械刺激缩足反应阈值(MWT).结果与基础值比较,Ⅲ组～Ⅵ组各时点MWT降低(P＜0.05),Ⅰ组和Ⅱ组各时点MWT差异无统计学意义(P＞0.05).与T2时比较,Ⅵ组T5～8时MWT升高(P＜0.05).与Ⅰ组和Ⅱ组比较,Ⅲ组～Ⅵ组MWT降低(P＜0.05)；与Ⅲ组、Ⅳ组和Ⅴ组比较,Ⅵ组T5～8时MWT升高(P＜0.05).结论脊髓CCL21参与了大鼠胫骨癌痛的维持.
목적 탐토척수추화인자배체21(CCL21)재대서경골암통중적작용.방법 건강성년자성SD대서48지,체중160～ 180 g,10～ 11주령,채용수궤수자표법,장대서수궤분위6조(n=8):가수술조(Ⅰ조)、가수술+ CCL21중화항체조(Ⅱ조)、골암통조(Ⅲ조)、골암통+용매PBS조(Ⅳ조)、골암통+대조IgG조(Ⅴ조)화골암통+CCI21중화항체조(Ⅵ조).채용경골골수강내주사Walker-256유선암세포방법제비경골암통모형.주사Walker-256유선암세포후14 d,Ⅳ조、Ⅴ조화Ⅵ조분별경L5,6초내주사PBS 15μl、친화순화적정상양IgG 10 μg( 15 μl)화CCL21중화항체10μg(15μl);Ⅱ조초내주사CCL21중화항체10 μg.우주사Walker-256유선암세포혹생리염수전1 d(기출상태)、주사후7、14 d급초내급약후0.5、1、2、4、8、12、24、48 h(T1～10)시측정궤계자격축족반응역치(MWT).결과 여기출치비교,Ⅲ조～Ⅵ조각시점MWT강저(P＜0.05),Ⅰ조화Ⅱ조각시점MWT차이무통계학의의(P＞0.05).여T2시비교,Ⅵ조T5～8시MWT승고(P＜0.05).여Ⅰ조화Ⅱ조비교,Ⅲ조～Ⅵ조MWT강저(P＜0.05)；여Ⅲ조、Ⅳ조화Ⅴ조비교,Ⅵ조T5～8시MWT승고(P＜0.05).결론 척수CCL21삼여료대서경골암통적유지.
Objective To investigate the role of chemokine ligand 21 (CCL21) in the spinal cord in tibia bone cancer pain (BCP) in rats.Methods Forty adult female SD rats weighing 160-180 g were randomly divided into 5 groups (n=8 each):sham operation group (group Ⅰ ); sham operation + CCL21 neutralizing antibody group (groupⅡ); BCP group (group [); BCP + PBS group (group Ⅳ); BCP + control IgG group (groupⅤ)and BCP + CCL21 neutralizing antibody group (group Ⅵ).BCP was induced by inoculating Walker-256 mammary gland carcinoma cells into the rat tibia medullary cavity in groups Ⅲ-Ⅵ.PBS 15 μl,IgG 10 μg and CCL21 neutralizing antibody 10 μg were injected intrathecally (IT) at 14 days after intra-tibial injection of Walker-256 mammary gland cancer cells in groups Ⅳ- Ⅵ respectively.Mechanical withdrawal threshold to yon Frey filament stimulation (MWT) was measured at 1 day before (To,baseline) ; 7 and 14 d after Walker-256 cell injection (T1,T2)and at 0.5,1,2,4,8,12,24 and 48 h after intrathecal injection (T3-10 ).Results Intra-tibial injection of Walker-256 mammary gland cancer cells significantly decreased MWT as compared with the baseline values in administration of CCL21 neutralizing antibody at T5-8 as compared with MWT before intrathecal administration at T2 in group Ⅵ.MWT was significantly lower in groups Ⅲ- Ⅳ than in groups Ⅰ and Ⅱ.MWT was significantly higher at T5-8 in group Ⅵ than in groups Ⅲ - Ⅴ.Conclu]sion CCL21 in the spinal cord is involved in the maintenance of tibia BCP in rats.