CAJ | 학술논문

目的研究准备抗病毒治疗的慢性丙型肝炎患者的免疫特点.方法将30例慢性丙型肝炎患者和10例正常对照外周血单个核细胞(PBMC)体外培养72 h后,用ELISA法检测培养上清中细胞因子IL-2、IL-4、IL-10、IL-12、IFN-γ和TNF-α的浓度.结果 (1)慢性丙型肝炎患者PBMC培养上清中IFN-γ、IL-10和TNF-α的水平明显升高(P＜0.05),没有检测到IL-2、IL-4、IL-12的基础分泌.(2)不同病情患者间的细胞因子的分泌水平差异无统计学意义(P＞0.05).结论慢性丙型肝炎患者体内TH2型细胞因子的分泌占优势.提示通过调整TH1/TH2失衡可能达到抗病毒治疗目的.
목적 연구준비항병독치료적만성병형간염환자적면역특점.방법 장30례만성병형간염환자화10례정상대조외주혈단개핵세포(PBMC)체외배양72 h후,용ELISA법검측배양상청중세포인자IL-2、IL-4、IL-10、IL-12、IFN-γ화TNF-α적농도.결과 (1)만성병형간염환자PBMC배양상청중IFN-γ、IL-10화TNF-α적수평명현승고(P＜0.05),몰유검측도IL-2、IL-4、IL-12적기출분비.(2)불동병정환자간적세포인자적분비수평차이무통계학의의(P＞0.05).결론 만성병형간염환자체내TH2형세포인자적분비점우세.제시통과조정TH1/TH2실형가능체도항병독치료목적.
Objective To study the immune state of patients with chronic hepatitis C before antiviral therapy by detecting the levels of the cytokines interleukin (IL)-2, IL-4, IL-10, IL-12, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α secretion by periferal blood mononuclear cells (PBMC). Methods The level of the cytokine secreted by PBMC in patients with chronic hepatitis C after culturing for 72 hours in vitro was detected by ELISA. Results (1) Compared with the cytokine level of normal controls, the level of IFN-γ, TNF-α, and IL-10 of patients with chronic hepatitis C significantly increased (P＜0.05) while IL-2, IL-4, and IL-12 were not detected in the culture supernatant of PBMC from both normal control and patients with chronic hepatitis C. (2) There was no statistically significant difference in secretion of the cytokines among patients with different severity of the disease. Conclusion PMBCs from patients with chronic hepatitis C tend to secret Th2 type cytokines. Restoring the imbalance of TH1/TH2 may help to clear the hepatitis C virus.