CAJ | 학술논문

目的观察溴隐亭对雌激素诱导Fisher344( F344)大鼠垂体泌乳素腺瘤的治疗作用及雌激素受体(ER)ERα、ERβ、多巴胺2型受体(D2R)的表达.方法采用免疫组织化学SP法检测泌乳素(PRL)、ERα、ERβ及D2R在垂体组织中的表达,放射免疫法检测血清PRL水平.结果模型组血清PRL水平(3579.8 ±412.5)μg/L与对照组(19.6±5.2) μg/L比较显著增高,治疗组(110.8±21.3) μg/L明显降低(P＜0.01).模型组与治疗组ERα的表达水平高于对照组；治疗组ERβ的表达水平高于另两组；D2R在3组中的表达差异无统计学意义(P＞0.05).结论溴隐亭可抑制雌激素诱导的F344大鼠泌乳素腺瘤生长及PRL分泌,具有治疗作用.ER可能在泌乳素腺瘤的发生发展中具有重要作用.
목적 관찰추은정대자격소유도Fisher344( F344)대서수체비유소선류적치료작용급자격소수체(ER)ERα、ERβ、다파알2형수체(D2R)적표체.방법 채용면역조직화학SP법검측비유소(PRL)、ERα、ERβ급D2R재수체조직중적표체,방사면역법검측혈청PRL수평.결과 모형조혈청PRL수평(3579.8 ±412.5)μg/L여대조조(19.6±5.2) μg/L비교현저증고,치료조(110.8±21.3) μg/L명현강저(P＜0.01).모형조여치료조ERα적표체수평고우대조조；치료조ERβ적표체수평고우령량조；D2R재3조중적표체차이무통계학의의(P＞0.05).결론 추은정가억제자격소유도적F344대서비유소선류생장급PRL분비,구유치료작용.ER가능재비유소선류적발생발전중구유중요작용.
Objective To investigate the therapeutic effect of bromocryptine on estrogen-induced pituitary prolactinomas in F344 rats and observe the expression levels of estrogen receptor (ER) ERα,ERβ and dopamine D2 receptor (D2R).Methods Prolactin (PRL),ERα,ERβ and D2 R expressions were detected by immunohistochemistry in pituitary gland.Radioimmunoassay was employed to detect serum PRL level.Results The Serum PRL level of the model group increased significantly compared with the control group,which were (3579.8 -±412.5) μg/L and ( 19.6 ±5.2) μg/L,separately.The serum PRL level ( 110.8 ± 21.3 ) μg/L of the therapeutic group was decreased ( P ＜ 0.01 ).The expression levels of ERα in prolactinomas of the model group and the therapeutic group were higher than the control group; ERβ expression level in the therapeutic group was higher than the other two groups.There were no differences in D2R expressions among the three groups (P ＞ 0.05 ).Conclusion Bromocryptine might have therapeutic effects on estrogen-induced pituitary prolactinoma through inhibiting PRL secretion and tumor growth.ER might play an important role in prolactinomas tumorigenesis and development.