CAJ | 학술논문

目的观察腹主动脉粥样硬化斑块中是否存在纳米细菌,并对其进行鉴定.方法 12例腹主动脉粥样硬化斑块及12例正常动脉血管组织研磨,取上清液培养.8周后收集培养瓶底白色粉末,用纳米细菌单克隆抗体间接免疫荧光法对其进行检测,并利用电镜进一步观察.结果 12例动脉粥样硬化斑块中10例纳米细菌检测阳性,2例阴性,12例正常动脉血管组织中1例纳米细菌检测阳性,11例阴性,差异有统计学意义(P＜0.05).纳米细菌单克隆抗体间接免疫荧光检测时可发出绿色荧光,电镜下测量纳米细菌直径约为100 ～ 300 nm.结论腹主动脉粥样硬化斑块中存在纳米细菌,它可能导致粥样硬化斑块不稳定.
목적 관찰복주동맥죽양경화반괴중시부존재납미세균,병대기진행감정.방법 12례복주동맥죽양경화반괴급12례정상동맥혈관조직연마,취상청액배양.8주후수집배양병저백색분말,용납미세균단극륭항체간접면역형광법대기진행검측,병이용전경진일보관찰.결과 12례동맥죽양경화반괴중10례납미세균검측양성,2례음성,12례정상동맥혈관조직중1례납미세균검측양성,11례음성,차이유통계학의의(P＜0.05).납미세균단극륭항체간접면역형광검측시가발출록색형광,전경하측량납미세균직경약위100 ～ 300 nm.결론 복주동맥죽양경화반괴중존재납미세균,타가능도치죽양경화반괴불은정.
Objective To investigate whether the nanobacteria exists in abdominal aorta atherosclerotic plaque or not and detect it.Methods 12 case of abdominal aorta atherosclerotic plaques and 12 case of normal artery were grinded,the supematant fluid was cultured for eight weeks.Subsequently,the white extract from the culture flask was detected by nanobacteria monoclonal antibody immunofluorescence and electron microscope.Results Nanobacteria was positive in 10 case of atherosclerotic plaques but only in 1 case of normal artery,the difference was statistically significant (P ＜ 0.05).Green fluorescence could be present during indirect immunofluorescence detection.The size of nanobacreria was measured ranging from 100nm to 300nm by electron microscope analysis.Conclusion Nanobacteria was detected in abdominal aorta atherosclerotic plaques and might led to atherosclerotic plaques instability.