CAJ | 학술논문

目的探讨氟对人正常甲状腺细胞Nthy-ori 3-1的毒性作用及机制.方法分别用0(对照)、0.1、1.0、3.0 mmol/L的NaF作用于体外培养的Nthy-ori 3-1细胞24h后,采用噻唑蓝染色法、乳酸脱氢酶(LDH)比色法检测存活率和LDH漏出率,采用流式细胞术检测活性氧(ROS)水平、细胞周期构成比及凋亡率.结果以对照组细胞存活率为100％,1.0、3.0 mmol/L NaF组细胞存活率分别为(76.64±9.13)％、(64.04±6.32)％,与对照组存活率的差异有统计学意义(P值均＜0.01).3.0 mmol/L NaF组LDH漏出率及ROS水平分别为(48.66±7.15)％,(29 993.50±1786.86),较对照组[分别为(35.24±3.02)％,(13 021.33±1067.55)]升高(P值均＜0.01).1.0 mmol/L NaF组细胞G0/G1期、S期细胞百分比分别为(40.76±5.65)％、(54.05±4.59)％,与对照组[分别为(60.09±1.76)％、(32.59±2.43)％]相比,差异有统计学意义(P值均＜0.01).3.0 mmol/L NaF组细胞凋亡率为(20.09±3.22)％,高于对照组[(9.64±3.44)％],P＜0.01.结论试验浓度的氟能降低甲状腺细胞存活率,增加LDH漏出率和ROS产生,并使细胞发生S期阻滞及诱导细胞凋亡.
목적 탐토불대인정상갑상선세포Nthy-ori 3-1적독성작용급궤제.방법 분별용0(대조)、0.1、1.0、3.0 mmol/L적NaF작용우체외배양적Nthy-ori 3-1세포24h후,채용새서람염색법、유산탈경매(LDH)비색법검측존활솔화LDH루출솔,채용류식세포술검측활성양(ROS)수평、세포주기구성비급조망솔.결과 이대조조세포존활솔위100％,1.0、3.0 mmol/L NaF조세포존활솔분별위(76.64±9.13)％、(64.04±6.32)％,여대조조존활솔적차이유통계학의의(P치균＜0.01).3.0 mmol/L NaF조LDH루출솔급ROS수평분별위(48.66±7.15)％,(29 993.50±1786.86),교대조조[분별위(35.24±3.02)％,(13 021.33±1067.55)]승고(P치균＜0.01).1.0 mmol/L NaF조세포G0/G1기、S기세포백분비분별위(40.76±5.65)％、(54.05±4.59)％,여대조조[분별위(60.09±1.76)％、(32.59±2.43)％]상비,차이유통계학의의(P치균＜0.01).3.0 mmol/L NaF조세포조망솔위(20.09±3.22)％,고우대조조[(9.64±3.44)％],P＜0.01.결론 시험농도적불능강저갑상선세포존활솔,증가LDH루출솔화ROS산생,병사세포발생S기조체급유도세포조망.
Objective To explore the toxic effect of fluoride on the human thyroid cells ( Nthy-ori 3-1 ) and its mechanism.Methods Nthy-ori 3-1 cells were exposed to 0.0,0.1,1.0,3.0 mmol/L of sodium fluoride (NaF) in vitro.After 24 hours incubation,3 (4,5-Dimethylthiazol-z-y1 )-3,5-diphenyltetrazolium bromide(MTT) assay and lactate dehydrogenase (LDH) assay were used to measure cell viability and the LDH leakage rate.Reactive oxygen species (ROS) level,constituent ratio of the cell cycle,and apoptosis rate were measured by flow cytometry.Results Comparing to viability of control group ( set as 100.00％ ),the cell viability of the 1.0,3.0 mmol/L fluoride-treated groups ( 76.64 ± 9.13 ) ％,(64.04 ±6.32)％ were significantly decreased (all P values ＜0.01 ).LDH leakage rate and ROS level of the 3.0 mmol/L fluoride-treated group ( (48.66+7.15)％,(29 993.50 ± 1786.86) FI) were significantly increased ( all P values ＜ 0.01 ) compared to control group ( ( 35.24 ± 3.02 ) ％,( 13 021.33 ± 1067.55 )FI).The G0/G1 phase cells of the 1.0 mmol/L fluoride-treated group ( (40.76 ±5.65)％ ) were lower than control group (60.09 ± 1.76)％ (P ＜ 0.01 ),yet the percentage of cells in S phase (( 54.05 ± 4.59 )％ )were higher than the control group ( 32.59 ± 2.43 ) ％ ( P ＜ 0.0l ).Comparing to control group ( ( 9.64 ±3.44) ％ ),the precentage of apoptosis cells increased in the 3.0 mmol/L fluoride-treated group ( (20.09 ±3.22)％) (P＜0.01).Conclusion To Nthy-ori 3-1 cells,fluoride under experimental concentrations decreases cell viability,improve the LDH leakage rate,and ROS leveL It blocks the cells in S phase and induce cell apoptosis.