肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2009年
5期
317-319
,共3页
韩振东%张忠太%任海%张永强%白学义
韓振東%張忠太%任海%張永彊%白學義
한진동%장충태%임해%장영강%백학의
食管肿瘤%不典型增生%Livin基因%荧光定量聚合酶链反应
食管腫瘤%不典型增生%Livin基因%熒光定量聚閤酶鏈反應
식관종류%불전형증생%Livin기인%형광정량취합매련반응
Esophageal neoplasms%Atypical hyperplasia%Livin gene%Real-time fluorescence quantitative PCR
目的 探讨食管癌发生、发展过程中凋亡抑制基因Livin的表达及其与食管癌临床病理的关系.方法 采用实时荧光定量聚合酶链反应(PCR)法,检测Livin在正常食管黏膜、不典型增生、原位癌及浸润癌组织中的表达强度.结果 Livin在正常食管黏膜、不典型增生、原位癌及浸润癌组织中呈渐进性高表达,分别为1.0、2.26±0.79、7.24±1.06、12.21±2.47,各组Livin mRNA表达水平总体均数不等(F=52.66.P<0.01).结论 Livin与食管癌的发生、发展密切相关.实时荧光定量PCR法检测食管癌中Livin基因,可作为食管癌早期诊断的可靠方法.
目的 探討食管癌髮生、髮展過程中凋亡抑製基因Livin的錶達及其與食管癌臨床病理的關繫.方法 採用實時熒光定量聚閤酶鏈反應(PCR)法,檢測Livin在正常食管黏膜、不典型增生、原位癌及浸潤癌組織中的錶達彊度.結果 Livin在正常食管黏膜、不典型增生、原位癌及浸潤癌組織中呈漸進性高錶達,分彆為1.0、2.26±0.79、7.24±1.06、12.21±2.47,各組Livin mRNA錶達水平總體均數不等(F=52.66.P<0.01).結論 Livin與食管癌的髮生、髮展密切相關.實時熒光定量PCR法檢測食管癌中Livin基因,可作為食管癌早期診斷的可靠方法.
목적 탐토식관암발생、발전과정중조망억제기인Livin적표체급기여식관암림상병리적관계.방법 채용실시형광정량취합매련반응(PCR)법,검측Livin재정상식관점막、불전형증생、원위암급침윤암조직중적표체강도.결과 Livin재정상식관점막、불전형증생、원위암급침윤암조직중정점진성고표체,분별위1.0、2.26±0.79、7.24±1.06、12.21±2.47,각조Livin mRNA표체수평총체균수불등(F=52.66.P<0.01).결론 Livin여식관암적발생、발전밀절상관.실시형광정량PCR법검측식관암중Livin기인,가작위식관암조기진단적가고방법.
Objective To detect the expression of apoptotic inhibitor gene Livin in different esophageal epithelial lesions and to analyze the relation between the expression of Livin with pathologic characteristics. Methods The expressions of Livin mRNA were detected by real-time fluorescence quantitative PCR in 40 patients with different esophageal epithelial lesions including normal, atypical hyperplasia, carcinoma in situ and invasive carcinoma. Results The expression of Livin was progressively increased from normal to invasive carcinoma. Conclusion The expression of Livin is significantly related to the progression of esophageal cancer. The detection of Livin by real-time fluorescence quantitative PCR may be a reliable means for early diagnosis of esophageal cancer.
