CAJ | 학술논문

目的　探讨细胞角蛋白基因13(CK13)和EB病毒DNA在人鼻咽癌(NPC)组织中的表达及其意义。方法　应用Northern杂交对32例NPC和8例慢性鼻咽炎(CINE)组织进行CK13 mRNA水平检测，同时应用PCR方法进行EB病毒DNA检测。结果　CK13基因在鼻咽炎组织中高表达，在NPC组织中的表达显著下调，下调的频率达65.63%(21/32)。结论　NPC组织的分化可能与CK13基因的表达下调有关。
목적　탐토세포각단백기인13(CK13)화EB병독DNA재인비인암(NPC)조직중적표체급기의의。방법　응용Northern잡교대32례NPC화8례만성비인염(CINE)조직진행CK13 mRNA수평검측，동시응용PCR방법진행EB병독DNA검측。결과　CK13기인재비인염조직중고표체，재NPC조직중적표체현저하조，하조적빈솔체65.63%(21/32)。결론　NPC조직적분화가능여CK13기인적표체하조유관。
Objective　To investigate the possible pathogenetic role of the Cytokeratin 13 (CK13) gene expression and Epstein-Barr virus (EBV) infection in human nasopharyngeal carcinoma (NPC) and their clinical implications. Methods　Tissue samples taken from a total of 32 cases of NPC and 8 cases of chronic inflammation of nasopharyngeal epithelium (CINE) were studied for the CK13 gene at the mRNA level by northern blot hybridization. Polymerase chain reaction (PCR) was used to detect EBV-DNA. Results　A high expression of the CK13 gene was found in 100% (8/8) of CINE, and a low and negative expression of the gene in 65.6% (21/32) of NPC. Conclusion　Most patients of NPC are poorly differentiated. This is possibly related to the down-regulation of CK13 gene expression.