CAJ | 학술논문

为了解环境胁迫对植物体中抗坏血酸含量及氧化还原状态的影响,以不同强度的冰冻和干旱两种胁迫为例,研究了它们对沈阳几种针叶树离体叶抗坏血酸、脱氢抗坏血酸含量以及抗坏血酸-谷胱甘肽循环中4种酶活性的影响.结果表明,两种胁迫达到一定强度后,都能使还原态抗坏血酸含量下降而使脱氢抗坏血酸含量上升.冰冻使抗坏血酸过氧化酶和单脱氢抗坏血酸还原酶活性下降.轻度失水使这两种酶活性上升,失水加重后转而趋于下降.脱氢抗坏血酸还原酶和谷胱甘肽还原酶活性对两种胁迫反应均不如前两种酶敏感.结合以前的研究结果,认为这一H2O2清除系统在导致驯化(acclimation)的轻度胁迫作用下可以得到加强,而当胁迫强度过大时则其清除能力下降并使组织受到伤害.文中还报告了沈阳几种针叶树抗寒性和针叶中抗坏血酸含量及上述4种酶活性之间的相关关系.
위료해배경협박대식물체중항배혈산함량급양화환원상태적영향,이불동강도적빙동화간한량충협박위례,연구료타문대침양궤충침협수리체협항배혈산、탈경항배혈산함량이급항배혈산-곡광감태순배중4충매활성적영향.결과표명,량충협박체도일정강도후,도능사환원태항배혈산함량하강이사탈경항배혈산함량상승.빙동사항배혈산과양화매화단탈경항배혈산환원매활성하강.경도실수사저량충매활성상승,실수가중후전이추우하강.탈경항배혈산환원매화곡광감태환원매활성대량충협박반응균불여전량충매민감.결합이전적연구결과,인위저일H2O2청제계통재도치순화(acclimation)적경도협박작용하가이득도가강,이당협박강도과대시칙기청제능력하강병사조직수도상해.문중환보고료침양궤충침협수항한성화침협중항배혈산함량급상술4충매활성지간적상관관계.
To investigate the effects of environmental stresses on ascorbic acid content and its redoxstatus, the effects of freezing and drought on ascorbate and dehydro-ascorbate content and activities of fourenzymes involved in the ascorbate-glutathione cycle in some conifers were studied. The results showed thatboth freezing and drought induced the decrease in ascorbate content and the increase in dehydro-ascorbatecontent. The activities of ascorbate peroxidase (APX) and monodehydro-ascorbate reductase (MDAR) weredecreased by freezing stress. At the beginning of exposure to air, water loss from detached needles inducedthe increase in the activities of APX and MDAR. Further water loss turned to decrease the APX and MDARactivities. The activities of dehydro-ascorbate reductase (DHAR) and glutathione reductase (GR) were notsensitive to changes in temperature and water content of the needles. It is concluded that moderate tempera-ture or water stresses may induce the acclimation and increase in the ability of the H2O2 scavenging system,while strong stresses decrease the ability and induce injury of plant tissues. Correlation between ascorbatecontent and activities of related enzymes and cold tolerance of conifers were also reported.