CAJ | 학술논문

目的研究氯高铁血红素对慢性肾衰竭大鼠血压的影响,并探讨其可能机制.方法用5/6肾切除法建立CRF模型,研究分为3组:(1)假手术组(sham组)、(2)慢性肾衰组(CRF组)、(3)氯高铁血红素组(hemin组).观察术后第10周(即给药后第8周)各组血清尿素氮、肌酐、尿蛋白、血红蛋白及术后第4,6,8和10周血压等指标;免疫组化方法检测肾组织中血红素氧化酶-1(HO-1)的表达和分布;双波长分光光度法测量血浆内源性CO的水平;放免法检测肾组织和血浆中内皮素-1(ET-1)的含量.结果与CRF组相比,hemin组在术后第6,8和10周均出现显著的血压下降(P＜0.05).同时,在术后第10周hemin组比CRF组血肌酐、尿素氮、尿蛋白显著降低(P＜0.05),贫血明显改善(P＜0.05);肾组织中HO-1表达明显增加,血浆中内源性CO水平明显升高,肾组织和血浆中ET-1水平明显降低(P＜0.05).结论hemin组具有延缓慢性肾功能衰竭大鼠高血压的发生和发展作用,这种作用与hemin诱导肾脏HO-1表达、增加血浆CO浓度,降低肾组织和血浆中的ET-1水平有关.
목적연구록고철혈홍소대만성신쇠갈대서혈압적영향,병탐토기가능궤제.방법용5/6신절제법건립CRF모형,연구분위3조:(1)가수술조(sham조)、(2)만성신쇠조(CRF조)、(3)록고철혈홍소조(hemin조).관찰술후제10주(즉급약후제8주)각조혈청뇨소담、기항、뇨단백、혈홍단백급술후제4,6,8화10주혈압등지표;면역조화방법검측신조직중혈홍소양화매-1(HO-1)적표체화분포;쌍파장분광광도법측량혈장내원성CO적수평;방면법검측신조직화혈장중내피소-1(ET-1)적함량.결과여CRF조상비,hemin조재술후제6,8화10주균출현현저적혈압하강(P＜0.05).동시,재술후제10주hemin조비CRF조혈기항、뇨소담、뇨단백현저강저(P＜0.05),빈혈명현개선(P＜0.05);신조직중HO-1표체명현증가,혈장중내원성CO수평명현승고,신조직화혈장중ET-1수평명현강저(P＜0.05).결론hemin조구유연완만성신공능쇠갈대서고혈압적발생화발전작용,저충작용여hemin유도신장HO-1표체、증가혈장CO농도,강저신조직화혈장중적ET-1수평유관.
[Objective] To study the effect of hemin on nephrogenic hypertension of chronic renal failure rats and its possible mechanism. [Methods] 5/6 renal nephrectomized rats were randomly assigned to three groups: sham operation group, CRF group and hemin group. We measured serum creatinine, BUN, urinary protein, HGB at the 10th week and blood pressure at the 4th, 6th, 8th, 10th week after operation. Immunohistochemistry was used to examine the expression and distribution of HO-1 in the rat kidney. The two-wavelength spectrophotometry was used to measure endogenous carbon monoxide in plasma. Radioimmunoassay was used to examine the level of ET-1 in the kidney and plasm. [Results] Compared with CRF group, nephrogenic hypertension of hemin group was reduced significantly at the 6th, 8th, 10th week after operation, and followed with the decrease of serum creatinine, BUN,urinary protein and the improvement of nephrogenic anemia at the 10th week after operation. Hemin not only increased the expression of HO-1, reduced the expression of ET-1 in the kidney, but also increased the concentration of carbon monoxide, reduced the level of ET-1 in the plasm. [Conclusion] Hemin can retard the occurrence and development of nephrogenic hypertension of chronic renal failure rats. This role has much to do with inducing the expression of HO-1 in the kidney, increasing the concentration of carbon monoxide in the plasm and reducing the level of ET-1 in the kidney and plasm.