CAJ | 학술논문

目的:建立HPLC丹皮酚胶囊血浓度测定方法,评价丹皮酚胶囊的药代动力学特点.方法:24名健康志愿者单剂量口服 160 mg 丹皮酚胶囊,按设定时间采集肘静脉血经乙睛萃取处理,以XB-C18 (250 mm×4.6 mm,5 μm) 色谱柱为固定相,四氢呋喃-甲醇-水-磷酸(6∶60∶34∶0.1) 为流动相测定丹皮酚血浆浓度.采用DAS2.0计算丹皮酚主要药动学参数.结果:丹皮酚线性范围10～500 ng/mL,最低检出浓度为 10 ng/mL.主要药代动力学参数:Cmax为(116±46) ng/mL, tmax为(1.02±0.13) h, t1/2为 1.03 h.结论:建立的HPLC方法特异性强、灵敏度高,可用于丹皮酚血药浓度测定和人体药动学研究.
목적:건립HPLC단피분효낭혈농도측정방법,평개단피분효낭적약대동역학특점.방법:24명건강지원자단제량구복 160 mg 단피분효낭,안설정시간채집주정맥혈경을정췌취처리,이XB-C18 (250 mm×4.6 mm,5 μm) 색보주위고정상,사경부남-갑순-수-린산(6∶60∶34∶0.1) 위류동상측정단피분혈장농도.채용DAS2.0계산단피분주요약동학삼수.결과:단피분선성범위10～500 ng/mL,최저검출농도위 10 ng/mL.주요약대동역학삼수:Cmax위(116±46) ng/mL, tmax위(1.02±0.13) h, t1/2위 1.03 h.결론:건립적HPLC방법특이성강、령민도고,가용우단피분혈약농도측정화인체약동학연구.
AIM: To establish a sensitive HPLC method for determining the concentrations of paeonol in human plasma and to evaluate its pharmacokinetic characteristics. METHODS: A single oral dose of 160 mg paeonol capsules was given to 24 Chinese healthy volunteers. Paeonol was separated on a XB-C18 column with tetrahydrofuran-methanol-water-phosphonic acid (6∶60∶34∶0.1, V∶V) as mobile phase. The plasma concentrations of paeonol were determined and its pharmacokinetic parameters were calculated and evaluated using DAS 2.0. RESULTS: The linear range of the paeonol was 10-500 ng/mL and the determination limit was 10 ng/mL. The main pharmacokinetic parameters, as Cmax, tmax, t1/2,AUC0-3, AUC0-∞ after a single dose of paeonol capsules were (116±46)ng/mL,(1.02±0.13) h,(1.03±0.35) h, (174±45) ng/mL, (217±56) ng/mL,respectively. CONCLUSION: The HPLC method for determining paeonol concentration in plasma is rapid, sensitive and suitable for pharmacokinetic studies.