植物病理学报
植物病理學報
식물병이학보
ACTA PHYTOPATHOLOGICA SINICA
2009年
5期
514-520
,共7页
陈夕军%王玲%左示敏%王子斌%陈宗祥%张亚芳%鲁国东%周而勋%郭泽建%黄世文%潘学彪
陳夕軍%王玲%左示敏%王子斌%陳宗祥%張亞芳%魯國東%週而勛%郭澤建%黃世文%潘學彪
진석군%왕령%좌시민%왕자빈%진종상%장아방%로국동%주이훈%곽택건%황세문%반학표
水稻纹枯病%抗性鉴定%鉴别品种%鉴别菌株%筛选
水稻紋枯病%抗性鑒定%鑒彆品種%鑒彆菌株%篩選
수도문고병%항성감정%감별품충%감별균주%사선
rice sheath blight%resistance identification%identification varieties%identification isolates%screening
经过近十年的病原菌接种试验,从国内外数百份水稻种质中,筛选出了抗病水平不同的5个代表品种Lemont、武育粳3号、Jasmine85、C418和YSBR1,作为水稻纹枯病菌致病力的鉴别品种.从江苏、广东、广西、海南、云南、湖南、福建等省采集、分离的水稻纹枯病菌中,初步筛选出致病力有差异的30个菌株,于温室苗期接种上述5个品种,确定了不同致病力的5个代表菌株C30、GD-118、E67、YN-7和YN-3.将选出的品种和菌株在江苏扬州和浙江富阳进行大田成株期抗性和致病力分析,结果表明:品种的抗病性和菌株的致病力都存在极显著差异,分别属于5个和3个不同的显著性级别,均可暂时作为鉴定材料使用.据以上结果,初步建立了一套可供水稻纹枯病菌致病力检测和寄主抗病性鉴定的鉴别体系.本文还讨论了建立水稻纹枯病鉴别体系的必要性、可行性和需要进一步开展的工作.
經過近十年的病原菌接種試驗,從國內外數百份水稻種質中,篩選齣瞭抗病水平不同的5箇代錶品種Lemont、武育粳3號、Jasmine85、C418和YSBR1,作為水稻紋枯病菌緻病力的鑒彆品種.從江囌、廣東、廣西、海南、雲南、湖南、福建等省採集、分離的水稻紋枯病菌中,初步篩選齣緻病力有差異的30箇菌株,于溫室苗期接種上述5箇品種,確定瞭不同緻病力的5箇代錶菌株C30、GD-118、E67、YN-7和YN-3.將選齣的品種和菌株在江囌颺州和浙江富暘進行大田成株期抗性和緻病力分析,結果錶明:品種的抗病性和菌株的緻病力都存在極顯著差異,分彆屬于5箇和3箇不同的顯著性級彆,均可暫時作為鑒定材料使用.據以上結果,初步建立瞭一套可供水稻紋枯病菌緻病力檢測和寄主抗病性鑒定的鑒彆體繫.本文還討論瞭建立水稻紋枯病鑒彆體繫的必要性、可行性和需要進一步開展的工作.
경과근십년적병원균접충시험,종국내외수백빈수도충질중,사선출료항병수평불동적5개대표품충Lemont、무육갱3호、Jasmine85、C418화YSBR1,작위수도문고병균치병력적감별품충.종강소、엄동、엄서、해남、운남、호남、복건등성채집、분리적수도문고병균중,초보사선출치병력유차이적30개균주,우온실묘기접충상술5개품충,학정료불동치병력적5개대표균주C30、GD-118、E67、YN-7화YN-3.장선출적품충화균주재강소양주화절강부양진행대전성주기항성화치병력분석,결과표명:품충적항병성화균주적치병력도존재겁현저차이,분별속우5개화3개불동적현저성급별,균가잠시작위감정재료사용.거이상결과,초보건립료일투가공수도문고병균치병력검측화기주항병성감정적감별체계.본문환토론료건립수도문고병감별체계적필요성、가행성화수요진일보개전적공작.
Five rice cultivars (YSBR1 ,C418,Jasmine85,Wuyujing3 and Lemont) with different levels of resistance to Rhizoctonia solani identified and screened from worldwide rice collections were selected to identify the pathogenicity of different isolates. Several hundred strains of R. solani had been isolated and purified from Jiangsu,Guangdong,Guangxi,Hainan, Yunnan,Hunan and Fujian Province. Thirty isolates with different path-ogenicity were selected to inoculate the five identification cultivars at the seedling stage in the greenhouse. Five isolates with obvious discrepancy of pathogenicity, C30,GD-118,E67, YN-7 and YN-3, were selected ulti-mately. The obtained cultivars and strains for identification were verified through field experiments at rice adult stage in Yangzhou of Jiangsu Province and Fuyang of Zhejiang Province. The results indicated that both the cultivars and isolates were divided significantly to five resistance grades and three pathogenicity grades respectively, so both of which could be used as identification materials. An identification system to the isolate pathogenity and the host resistance for rice sheath blight was preliminarily setup. The necessity and feasibility about the identification system, and the further work were also discussed in the paper.
