CAJ | 학술논문

NTPDase(Nucleoside triphosphate-diphosphohydrolase,核苷酸双磷酸酶)在哺乳动物巾分布于细胞膜,可以水解细胞外ATP和其他核苷酸,从而调控胞外核苷酸代谢及信号应答.根据水稻NTPD基因序列设计引物,在不同胁迫条件样品中,通过PCR扩增水稻NTPD基因,结果表明水稻NTPD基因表达水平在盐胁迫样品中升高.为进一步分析水稻NTPD基因的功能,构建了该基因的过量表达、RNA干涉载体,通过根癌农杆菌介导转入水稻品种日本晴并获得阳性植株.半定量RT-PCR分析达到过量表达和干涉日的.在盐胁迫条件下,TO代过表达转基因植株表现出一定程度的抗胁迫能力,RNA干涉转基因植株的生长则受到抑制.图7表3参20
NTPDase(Nucleoside triphosphate-diphosphohydrolase,핵감산쌍린산매)재포유동물건분포우세포막,가이수해세포외ATP화기타핵감산,종이조공포외핵감산대사급신호응답.근거수도NTPD기인서렬설계인물,재불동협박조건양품중,통과PCR확증수도NTPD기인,결과표명수도NTPD기인표체수평재염협박양품중승고.위진일보분석수도NTPD기인적공능,구건료해기인적과량표체、RNA간섭재체,통과근암농간균개도전입수도품충일본청병획득양성식주.반정량RT-PCR분석체도과량표체화간섭일적.재염협박조건하,TO대과표체전기인식주표현출일정정도적항협박능력,RNA간섭전기인식주적생장칙수도억제.도7표3삼20
In mammalian, nucleoside triphosphate-diphosphohydrolases (NTPD) is anchored in the plasma to hydrolyze ectonucleoside ATP and other nucleosides. Thus, NTPD can control the concentration of extracellular nucleotides to regulate nucleotides metabolism, as well as signal response. In the present study, according to the sequence of OsNTPD, the primers were designed and the full-length sequence of OsNTPD was amplified by PCR in Nipponbare with different treatments. It was shown that the expression levels of OsNTPD increased under salt retreatment. To identify the function of OsNTPD, the over-expressing and RNA-interference vectors of OsNTPD were constructed and introduced into rice {Oryza sativa L. cv. Nipponbare) by Agrobacterium-mediated transformation. The semi-quantitative RT-PCR revealed an increase of OsNTPD expression levels in the over-expressing transgenic plants and a significant reduction in the RNAi transgenic plants. Under salt stress, TO over-expressing transgenic plants showed more or less resistance, whereas the growth of RNAi transgenic rice plants was suppressed. Fig 7, Tab 3, Ref 20